BACKGROUND/AIM Corneal graft survival depends critically on the quality of the endothelium. In this study the authors aimed to evaluate corneal endothelium in mice at different times after transplantation and to correlate endothelial integrity with corneal graft survival. METHODS Syngeneic and allogeneic corneal grafts at various times (days 0-60) after engraftment were examined in flat mount...

purpose: congenital corneal leukoma is rare with an incidence of 6/100000 and is one of the most important causes of amblyopia. here we report an unusual case of bilateral congenital elevated corneal leukoma. methods: we encountered a 13-day-old full term male newborn. ocular examination of both eyes revealed central elevated corneal leukomas with a narrow normal lucid interval to limbus. corne...

The authors compared cytoskeletal elements of the in situ human trabecular-meshwork cell with in situ human corneal cells using indirect immunofluorescence staining for tubulin and intermediate filaments (vimentin, cytokeratin, and desmin) and NBD-phallacidin staining for f-actin using both fixed frozen and unfixed frozen sections from postmortem eyes. Both f-actin and tubulin were found throug...

PURPOSE The ultrastructure of tight junctions in the corneal endothelium has been studied extensively, yet little is known about their molecular composition. Junctional adhesion molecule-A (JAM-A) is a tight junction-associated adhesion protein previously implicated in tight junction assembly and regulation of barrier function. In this study, we sought to investigate the expression and function...

purpose: to report a case of anterior segment eye deposits in a patient on long-term chlorpromazine therapy. case report: a 34 years old man had been on chlorpromazine therapy for 5 years with a total dose of 1,100 gram. since one year ago, he presented with gradual onset of decreased visual acuity in both eyes. slit-lamp examination revealed multiple fine yellow-brown deposits on the corneal e...

PURPOSE Carbonic anhydrases play a central buffering role in current models of fluid transport in corneal endothelium, but in humans, clinical use of carbonic anhydrase inhibitors (CAIs) for the management of glaucoma does not cause corneal swelling. This study compares species differences in response to CAIs in human versus bovine corneal endothelial transport. METHODS Short-circuit current ...

deep anterior lamellar keratoplasty (dalk) has recently been introduced as an alternative procedure to penetrating keratoplasty (pk) for corneal pathologies not affecting the corneal endothelium. dalk does not rely on donor endothelium and requires less rigid criteria for donor corneal tissue quality. therefore, dalk makes it possible to use donor corneas deemed unsuitable for pk. furthermore, ...

PURPOSE To examine the involvement of p27(KIP1) in the regulation of the proliferation of the developing corneal endothelium. METHODS Central and peripheral corneas in C57Bl6 mice at postnatal day (P)1, P11, and 12 weeks after birth were analyzed by immunocytochemistry with anti-p27(KIP1), -p57(KIP2), and -proliferating cell nuclear antigen (PCNA) antibodies. Nuclear staining was performed wi...

PURPOSE To evaluate the functional outcome of tissue-engineered corneal endothelium reconstructed on a devitalized carrier and transplanted in the living feline model. METHODS Eighteen healthy adult cats underwent full-thickness corneal transplantation. In 11 animals, the donor cornea was reconstructed from cultured allogeneic feline corneal endothelial cells seeded on the denuded Descemet's ...

The corneal endothelium maintains corneal transparency by its pump and barrier functions; consequently, its decompensation due to any pathological reason causes severe vision loss due to corneal haziness. Corneal transplantation is the only therapeutic choice for treating corneal endothelial dysfunction, but associated problems, such as a shortages of donor corneas, the difficulty of the surgic...