Here, we demonstrate self-organized formation of apico-basally polarized cortical tissues from ESCs using an efficient three-dimensional aggregation culture (SFEBq culture). The generated cortical neurons are functional, transplantable, and capable of forming proper long-range connections in vivo and in vitro. The regional identity of the generated pallial tissues can be selectively controlled ...

Background Cryopreservation of ovarian cortex is increasingly used to preserve fertility before cancer therapy. Recently, studies show that Brain-derived neurotrophic factor (BDNF) may be involved in oocyte maturation. Brain-derived neurotrophic factor (BDNF) is member of neurotrophin family that has anti-apoptotic effects on nervous system. Recent researches show that it also plays key role in...

Astrocytes are an abundant cell type in the mammalian brain, yet much remains to be learned about their molecular and functional characteristics. In vitro astrocyte cell culture systems can be used to study the biological functions of these glial cells in detail. This video protocol shows how to obtain pure astrocytes by isolation and culture of mixed cortical cells of mouse pups. The method is...

The central neurotoxicity of the excitatory amino acid neurotransmitter glutamate has been postulated to participate in the pathogenesis of the neuronal cell loss associated with several neurological disease states, but the complexity of the intact nervous system has impeded detailed analysis of the phenomenon. In the present study, glutamate neurotoxicity was studied with novel precision in di...

hydronephrosis occurs as a congenital or an acquired condition following obstruction of the urinary tract. in this study, a four month old male holstein calf with emaciation, growth retardation and a poor dry scruffy hair coat was examined because of remarkable distention of right abdomen. at necropsy, right kidney was hydronephrotic as a very big fluid-filled round pelvis with the presence of ...

aneurysmal bone cyst (abc) is a benign expansile bone tumor, most commonly involving the medulla of long bones. abc rarely arises within the cortex or in the subperiosteal region, radiographically mimicking other conditions, in particular surface osteosarcomathat is low-grade in nature and may go secondary abc changes, and telangiectatic osteosarcoma. both of these are sometimes mistaken micros...

intracranial lipomas are congenital malformations. these uncommon lesions have an incidence of 0.1 to 1.7% of all intracranial tumors. most cases are located at midline and 5% are along the sylvian fissures. if symptomatic, seizures are the most common symptom. these tumors are slow growing and have favorable outcome. we report a case of a 25-year-old man whose ct and mri revealed a lesion in r...

there have been a number of developments in screw design and implantation techniques over recent years, including proposal of an alternative trajectory entitled as cortical bone trajectory (cbt). cortical bone trajectory has been investigated in recent medical treatments as an alternative for screw fixation aimed at increasing purchase of pedicle screws in higher density bone. cbt screw inserti...

background: bisphosphonates (bps) are used extensively for managing the osteoporosis. there are some controversies on atypical fractures of femur that associated with increase in cortical thickness (ct) and bps' use. in this study, the effects of alendronate consumption were studied on femoral ct as a predictor for atypical fracture.   methods: forty nine post-menopausal women aged 50-70 years ...