Purpose : To evaluate the effectiveness and expediency of using vesicles outer membranes atoxygenic strains Vibrio cholerae O1 serogroup for prevention experimental cholera. Materials methods atoxigenic V. El Tor 18950 (ctxAB-tcpA-) 18780 (ctxAB-tcpA+) isolated from water surface reservoirs were used to obtain vesicles. The protective properties obtained drugs evaluated a model generalized chol...

We initially attempted to isolate a Vibrio cholerae O1 El Tor biotype that carries a novel variant of the cholera toxin gene (ctxAB) from environmental waters of Indonesia, where the seventh cholera pandemic by V. cholerae O1 El Tor biotype began. Nested PCR targeting the gene revealed that a total of eight strains were found to carry ctxAB. However, sequencing of the 16S rRNA genes of these is...

Cholera is an intestinal infection caused by Vibrio cholerae. The hallmark of the disease is profuse secretory diarrhea. Cholera can be endemic, epidemic, or pandemic. A total of 15 Vibrio cholerae isolates were obtained from diarrheal samples by standard bacteriological and serological methods. After DNA extraction, a polymerase chain reaction (PCR) assay was performed for detection of the pre...

The cholera toxin (CT) genes ctxAB are carried on a lysogenic phage of Vibrio cholerae, CTXPhi, which can transfer ctxAB between toxigenic and nontoxigenic strains of bacteria. This transfer may pose a problem when live oral cholera vaccine is given to people in epidemic areas, because the toxin genes can be reacquired by the vaccine strains. To address this problem, we have constructed a live ...

OBJECTIVE To investigate whether the HlyA-induced vacuolating effect is produced by V. cholerae O1 ElTor strains isolated from different geographic origins, including Mexico. MATERIAL AND METHODS Supernatant-induced haemolysis, vacuolating activity and cytotoxicity in Vero cells were recorded. PCR, RFLP analysis and molecular cloning were performed. RESULTS All ElTor strains analyzed induce...

Between November 2010 and April 2011, 11 cases of cholera were identified and associated with the consumption of raw oysters harvested from Apalachicola Bay, Florida. The etiological agent was the ctxAB-positive Vibrio cholerae serogroup O75. The genome sequences of the isolates provide useful information and are deposited in the public genome databases.

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...

Cholera is a life-threatening diarrhoeal disease, which mainly affects inhabitants of developing countries due to poor socio-economic conditions and lack of access to potable water and sanitation. Toxigenic Vibrio cholerae are the aetiological agents of cholera. These bacteria are autochthonous to aquatic environments, hence water plays a central role both in the epidemiology and transmission o...

The isolates of Vibrio cholerae (Vc) non-O1 from the Sudanese outbreak in 1968 produce an enterotoxin similar to cholera toxin (CT) of Vc O1. However, we have observed that the subunit B of CT purified from a Sudanese isolate (strain $7) was a larger oligomer than that of Vc O1 (Yamamoto et al., 1983). This larger oligomer formation was suggested to be due to difference in the subunit B from CT...