Received for publication 8 December 1965. reduced copper is oxidized to cupric ions by means of iodine. If no necessary allowance has been made, the cupric ions formed would be completely reduced and precipitated as cuprous iodide in the presence of an excess of soluble iodide in the reagent. Probably to check this reduction by iodide, Harding and Downs (1933) employed potassium oxalate in thei...

The binding of cupric or zinc ion by sperm whale metmyoglobin appears to involve imidazole groups of histidyl residues (4, 5) that occur well removed from the NH2 terminus (6). The binding of each cupric ion by metmyoglobin is accompanied on the average by the displacement of one or more protons beyond that that may be borne by the imidazole group, presumably from peptide amide nitrogen atoms (...

background: combination of glycation and oxidation is associated with diabetes mellitus. the aim of this study was to clarify the effect of glycated proteins in presence of transition metal ions on production of reactive oxygen species (ros) in rat hepatocyte suspension.  methods: glycated albumin was prepared by incubation of bovine serum albumin with 100 mm glucose in 0.3 m phosphate buffer a...

Interactions between carnosine (beta-alanyl-L-histidine), being plentiful in skeletal muscles and neuronal tissues, and captopril, a widely used angiotensin-converting enzyme (ACE) inhibitor, were examined concerning free radical scavenging activity and ACE activity in vitro. Not only captopril, but also carnosine, at concentrations less than those ordinarily found in muscles and neuronal tissu...

Apparently free-base cytokinins can interact with cupric ions in a specific manner. Oxidation of NADH by a horseradish peroxidase system was strongly promoted by such cytokinins provided cupric ions were present. Oxidation was promoted by 5 micromolar kinetin, zeatin, 6-benzylaminopurine (BA), or 6-(Delta(2)-isopentenylamino)purine (2iP) but not by adenine, 6-methylaminopurine or 6,6-dimethylam...

Background: Combination of glycation and oxidation is associated with diabetes mellitus. The aim of this study was to clarify the effect of glycated proteins in presence of transition metal ions on production of reactive oxygen species (ROS) in rat hepatocyte suspension.  Methods: Glycated albumin was prepared by incubation of bovine serum albumin with 100 mM glucose in 0.3 M phosphate buffer a...

The mechanisms and spectroscopic properties generated by intermediate states upon cupric ion binding to flexible peptide motifs in proteins are of considerable interest. One such motif is the Cys-X-X-X-Cys motif characteristic to members of the Sco family of proteins. In the antibiotic producing bacterium, Streptomyces lividans, a role for its Sco protein (Sco(Sl)) as a cupric metallochaperone ...

Novel chemical and biological sensors based on a single poly(pyrrole)-NTA chelator nanotube for sensitive, selective, rapid and real-time detection of histidine-tagged protein and cupric ions are reported. NTA groups on the nanotube surface provided a simple mechanism for metal ion sensing via the high-affinity interaction between NTA and the subsequent detection of histidine-tagged protein thr...