An electrochemical in vivo flow-injection system with an on-line microdialysis sampling is proposed for the simultaneous monitoring of L-lactate and glucose in rat brain. In the first stage of the operation, the dialysate from the microdialysis probe is delivered to a sample loop of the six-way autoinjector by perfusing Ringer's solution for 80 s at 5 microl min(-1). In the second stage, the di...

The term 'ultrapure water' is a common way to define water used for on-line treatments: it refers to the absence of chemical, organic and microbiological contamination. To be more accurate in definition, every known and potential contaminant has to be fixed at its limit value. AAMI recommendations and various Pharmacopoeias have set limits for dialysate based on traditional dialysis treatments,...

background protein-bound toxins such as indoxyl sulfate (is) are not efficiently removed by conventional hemodialysis (hd). objectives to improve the removal of is, we performed an in vitro study to evaluate the effects of high dissolved hydrogen on the dissociation of is from albumin using simulated hd. materials and methods wasted dialysate from peritoneal dialysis was concentrated a hundred ...

The dialysate calcium (Ca) concentration for hemodialysis (HD) patients can be adjusted to manage more optimally the body's Ca and phosphate balance, and thus improve bone metabolism as well as reduce accelerated arteriosclerosis and cardiovascular mortality. The appropriate dialysate Ca concentration allowing this balance should be prescribed to each individual patient depending on a multitude...

AIM In this work we estimated the contribution of the fluorescence of 4-pyridoxic acid (4-PA) to the total fluorescence of spent dialysate with the aim of evaluating the on-line monitoring of removal of this vitamin B-6 metabolite from the blood of patients with end-stage renal disease (ESRD). METHODS Spectrofluorometric analysis of spent dialysate, collected from hemodialysis and hemodiafilt...

BACKGROUND An important advantage of convective therapies is improved vascular reactivity. However, it is not well known whether the vascular response during convective therapies remains superior when compared to haemodialysis (HD) with an adjusted temperature of the dialysate. It has also been suggested that convective therapies may impair small electrolyte removal through an effect on the Don...