conclusions the results revealed that the novel multi-epitope dna construct could be an effective candidate in tuberculosis vaccine development, and it is qualified to investigate its potential to induce cd4 and cd8 t- cell immune response in the experimental animal model. results mhc class i-and class ii-predicted epitopes showed a high potential to binding to human hlas alleles, with global b...

DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...

It has been recognized that there is a need for a language mechanism that support reuse of collections of related classes. Existing approaches either use an enclosing class for this purpose, or introduce a special purpose, new language construct. In this paper we explore the use of packages for the grouping of related classes. Ordinary packages are already grouping of classes, so we explore the...

Background: Cell mediated immunity, especially cytotoxic T cell responses against HIV-1 infection, plays a critical role in controlling viral replication and disease progres-sion. DNA vaccine is a novel technology which is known to stimulate strong cellular immune responses. Many DNA vaccines have been tested for HIV infection but there is still no effective vaccine against this infection. Cons...

We introduce and study a tactic language, Hitac, for constructing hierarchical proofs, known as hiproofs. The idea of hiproofs is to superimpose a labelled hierarchical nesting on an ordinary proof tree. The labels and nesting are used to describe the organisation of the proof, typically relating to its construction process. This can be useful for understanding and navigating the proof. Tactics...

To construct of an eukaryotic expression vector encoding herpes simplex virus type 2 (HSV-2) glycoprotein D (gD2), an Iranian isolate of HSV-2 was propagated in HeLa cell line and its DNA was extracted and used as template in polymerase chain reactions (PCR), to amplify gD2 gene. Primers were designed and the restriction enzyme sites for EcoRI and XhoI were considered at their 5′ ends respectiv...

Recent developments in biofunctional DNA hydrogels and nanocomplexes based on rolling circle amplification (RCA) are described assembly strategies functionalization methods of the ultralong single-strand produced by RCA to construct materials introduced Review article Yang, Yao et al. (DOI: 10.1002/chem.202202673).