In this research, some independent experiments were carried out for investigating the effects of microspore developmental stage, bud pretreatments (cold, heat and Ca), and embryo induction medium on anther culture of some strawberry cultivars. The results showed that the percentages of responsive and embryogenic anthers in cultivar Paros in liquid medium were influenced not only by different de...

A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledon...

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

the aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. blastocyst stage embryos from pregnant nmri mice were obtained and cultured for 1-2 days in dmem medium. the inner cell masses formed...

the major objective of seedless grape breeding program is production of grapes with high quality. breeding of seedless grapes is difficult because the embryos are aborted after fertilization. embryo rescue technique is utilized to obtain plants from crosses between seedless cultivars. in the present study, the effect of paternal genotypes and different iaa concentrations in presence of ba appli...

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...

optimization of regeneration methods in cotton is necessary to improve and successful production of transgenic cotton cultivars by tissue culture technique. this study was conducted to determine the genotypic effect on callus induction in cotton using 3 genotypes (coker349, 4-s-4 and c1211) and coker 312 selected and a study was carried out in cotton research institute of gorgan using a factori...

Highly reproducible and simple protocol for cotton somatic embryogenesis is described here by using different concentrations of maltose, glucose, sucrose and fructose. Maltose (30 g/l) is the best carbon source for embryogenic callus induction and glucose (30 g/l) was suitable for induction, maturation of embryoids and plant regeneration. Creamy white embryogenic calli of hypocotyl explants wer...

An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...

Sorghum is a wonder crop from physiological point of view. It is the most important cereal crop, after rice and wheat.  The number of reports describing the use of transgenic Sorghum for basic studies in Biotechnology is still limited when compared to other crops. In one hand, the success of the transformation techniques is mainly dependent upon the availability of optimal protocols for highly ...