The ammonium sulfate coprecipitation technique of Farr was applied in a study of the purified enterotoxins of Staphylococcus aureus. Ammonium sulfate coprecipitation of iodine-131-labeled enterotoxins A, B, and C, with the use of a 1.6 m concentration of (NH(4))(2)SO(4), revealed differences in the antigen-binding capacity of normal and immune rabbit sera for the enterotoxins. The coprecipitati...

Purified Staphylococcus aureus enterotoxins B and C(1), the causative agents of staphylococcal food poisoning, were resolved into several components by scanning isoelectric focusing in sucrose density gradients in the absence and presence of 6 M urea and 5 mM dithiothreitol. The observed heterogeneity persisted in the denaturing medium, which suggests that the primary structure of protein enter...

The direct and indirect effects of staphylococcal enterotoxins A and C on the flux of water, sodium, and potassium have been studied in paired Thiry Vella fistulae in dogs. Administration of toxin resulted in a significant decrease in absorption, both in the loop to which the toxin had been administered and its pair. This decrease in absorption was associated with a decrease in movement out of ...

Staphylococcal food poisoning is one of the most prevalent causes foodborne intoxication worldwide. Sandwiches and desserts are susceptible to contamination by S. aureus due high proportion manual work during their production. Our study aimed evaluate impact storage conditions on staphylococcal enterotoxin production in sandwiches buttercream puffs. Foods were inoculated with different strains ...

Sera from 33 persons with staphylococcal infections and from 37 healthy persons were surveyed for the presence of antibody to staphylococcal enterotoxins A, B, C, D, and E and toxic shock syndrome toxin 1. Thirty-one (93.9%) of the patients and 35 (94.6%) of the control group had antibodies to one or more of the enterotoxins. The numbers of patients with antibody to the enterotoxins were as fol...

Foodborne Diseases (FBD) affect millions of people worldwide. They are caused mainly by bacteria the genus Staphylococcus, Staphylococcus aureus. Among these, some produce enterotoxins that resistant to high temperatures and can contaminate various types foods such as milk dairy products, in addition meat products others. For these reasons, it is necessary study S aureus Staphylococcal Enteroto...

Staphylococci are a worldwide cause of human and animal infections including life-threatening cases of bacteraemia, wound infections, pyogenic lesions, and mastitis. Enterotoxins produced by some staphylococcal species were recognized as causative agents of staphylococcal food poisoning (SFP), being also able to interrupt human and animal immune responses. Only enterotoxins produced by Staphylo...

Animal diseases due to enterotoxigenic Escherichia coli (ETEC) typically appear as severe watery diarrhoea during the first few days of life (also a few days after weaning in pigs). ETEC adhere to the small intestinal microvilli without inducing morphological lesions and produce enterotoxins acting locally on enterocytes. This action results in the hypersecretion (of water and electrolytes) and...

Staphylococcus aureus (S. aureus) is a Gram positive bacterium that is carried by about one third of the general population and is responsible for common and serious diseases. These diseases include food poisoning and toxic shock syndrome, which are caused by exotoxins produced by S. aureus. Of the more than 20 Staphylococcal enterotoxins, SEA and SEB are the best characterized and are also reg...