Immobilization of Lipase produced from Rhizomucor miehei on HDPE fine powder was investigated. As compared to an aqueous system, immobilization in a non-aquous organic medium such as n-hexane was not successful and caused enzyme denaturation. Prewetting the support with ethanol increased the immobilized protein and enzyme activity as much as 31% and 34%, respectively. The maximum immobilized a...

In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of  the stability of the resulted sulfite biosensor revealed that formation of a passive...

Slurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). Susceptibility of the samples to cellulose-hydrolysis increased initially with pretreatment time, reaching a maximum or a constant level thereafter. Maximum glucose yi...

Biological treatment, especially enzymatic methods, can be employed for effective and environmental- friendly treatment of dye effluents. Laccase, belonging to the blue multi-copper oxidases category, can oxidize a wide variety of substrates, especially synthetic dyes. In this study, laccase was used to biodegrade two azo dyes, i.e., Direct Red 23 and Acid Blue 92. Before conducting the exp...

In this study, immobilization of partially purified almond (Amygdalus communis) β-galactosidase on Con A layered calcium alginate-cellulose beads was investigated. Immobilized β-galactosidase retained 72% of theinitial activity after crosslinking by glutaraldehyde. Both soluble and immobilized enzyme exhibited the samepH and temperature optima at pH 5.5 and 50ºC, respectively. Howev...