Metastasis is a key factor that limits survival in the majority of patients with cancer. Thus, numerous efforts have been made to elucidate the molecular mechanisms involved in this phenomenon. B16‑F10 melanoma cells have been demonstrated to be highly metastatic to the lungs in mice. The aim of the current study was to investigate the role of CXC motif chemokine receptor 4 (CXCR4) in the metas...

The F10 is a new commercially available scanning laser confocal ophthalmoscope (SLO) that can perform multiple functions. We determined the usefulness of noninvasive evaluation of proliferative diabetic retinopathy (PDR) pathologies before and after intravitreal injection of bevacizumab (IVB) using the new indirect viewing system of the retro-mode function of the F10 SLO, and compared the image...

Background: Expression of the small peptide thymosin 4 is associated with angiogenesis induction, accelerated wound healing, and the metastatic potential of tumor cells. However, little is known about the mechanism(s) by which thymosin 4 promotes metastasis. Methods: Northern blot analysis and immunohistochemistry were used to examine thymosin 4 expression in mouse melanoma B16 cell lines and i...

Several cell surface properties of three B16 melanoma var iant lines, which exhibit differing lung-colonizing capacities, have been examined. Equal numbers of cells from the B16F10Lr and B16-F1 lines, injected i.v. into syngeneic mice, produced significantly fewer lung nodules than did cells of the B16-F10 line. These wide differences in biological behavior could not, however, be ascribed to ma...

Several cell surface properties of three B16 melanoma var iant lines, which exhibit differing lung-colonizing capacities, have been examined. Equal numbers of cells from the B16F10Lr and B16-F1 lines, injected i.v. into syngeneic mice, produced significantly fewer lung nodules than did cells of the B16-F10 line. These wide differences in biological behavior could not, however, be ascribed to ma...

Intratumoral regions of low extracellular pH and low nutrition are common features of solid tumors. Although cancer cells normally die when they are removed from their environment, a small population of cells survive. In the present study, the subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH/low nutrient-resis...

The effect of glucose in a modified Ham's F10 medium (MM) without hypoxanthine, phosphate and transition metals on human fertilization and sperm survival in vitro was determined. Mature human oocytes from in-vitro fertilization (IVF) patients or Percoll-washed human spermatozoa were randomly allocated to one of the treatment groups: normal Ham's F10, MM, MM with 5 mM glucose (HGMM) and MM with ...

The aim of this study was to evaluate the viability of bull spermatozoa diluted with commercial semen extender and two culture media stored at controlled room temperature (24 °C) for 72 hours. Two Nguni bulls were used for semen collection with the aid of an electro-ejaculator. After macroscopic evaluation, semen was pooled and aliquoted randomly into Triladyl, modified Ham’s F10, and TCM-199 c...

Two murine monoclonal antibodies (MAb), 2C5-F10 and 8D1-H10, reactive with Escherichia coli O4 and H5 antigens, respectively, were generated and characterized. Enzyme immunoassays and immunoblots demonstrated that MAb 2C5-F10 reacted specifically with lipopolysaccharide O antigen of E. coli O4 isolates, while MAb 8D1-H10 reacted with E. coli strains expressing H5 flagella.

We describe the complete factorization of the tenth Fermat number F10 by the elliptic curve method (ECM). F10 is a product of four prime factors with 8, 10, 40 and 252 decimal digits. The 40-digit factor was found after about 140 Mflop-years of computation. We also discuss the complete factorization of other Fermat numbers by ECM, and summarize the factorizations