background: we evaluated the genetic diversity of samples identified morphologically as fasciola spp. from sheep, cattle and goat from kermanshah province, western iran using pcr-rflp method. methods: we used pcr-rflp analysis of ribosomal its1 fragment using rsai restriction enzyme to investigate the genetic characteristics of fasciola species obtained from different hosts (16 sheep, 28 cattle,...

BACKGROUND Fascioliasis, caused by Fasciola hepatica and F. gigantica, has medical and economic importance in the world. Molecular approaches comparing traditional methods using for identification and characterization of Fasciola spp. are precise and reliable. The aims of current study were molecular characterization of Fasciola spp. in West Azerbaijan Province, Iran and then comparative analys...

  Objective(s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica , based on profiles of RFLP, produced by effect of endo...

OBJECTIVE(S) Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on I...

Eosinophilia is considered as severe at levels of >5 × 10 9 /L [1]. In childhood, the causes of severe eosinophilia are parasitic infections A boy from a rural area of Turkey, aged 6 years and 10 months, was admitted to our clinic with abdominal pain and severe hypereosinophilia. He had lately developed abdominal pain at the umbilical area, waxing and waning in character. His physical examinati...

Fascioliasis is a zoonotic infection caused by Fasciola hepatica. It is rarely seen with icterus caused by obstruction of the common bile duct. We report five patients with obstructive jaundice due to Fasciola hepatica, who were diagnosed and managed with endoscopic retrograde cholangiopancreatography (ERCP). All cases were admitted to hospital with complaints of icterus and pain in the right u...

An evaluation of lesions in the livers of Black and White cattle, aged 4 to 7, naturally infested with Fasciola hepatica was performed. The studies included a comprehensive analysis of histological lesions and liver enzyme activities determined with histochemical methods depending on the degree of natural Fasciola hepatica infestation. The studies revealed increased number of reticular fibres, ...

BACKGROUND The aim of this study was to compare superoxide dismutase (SOD) activity in Fasciola hepatica and Fasciola gigantica parasites. MATERIALS F. gigantica and F. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °C. Excretory-Secretory (ES) products were collected, centrifuged and stored at -20(°)C. E-S protein concentration was measured by Brad...