MOTIVATION Large-scale functional genomics analysis is now feasible and presents significant challenges in data analysis, storage and querying. Data standards are required to enable the development of public data repositories and to improve data sharing. There is an established data format for microarrays (microarray gene expression markup language, MAGE-ML) and a draft standard for proteomics ...

Formylglycine-generating enzyme (FGE) is an O2 -utilizing oxidase that converts specific cysteine residues of client proteins to formylglycine. We show that CuI is an integral cofactor of this enzyme and binds with high affinity (KD =of 10-17  m) to a pair of active-site cysteines. These findings establish FGE as a novel type of copper enzyme.

Fluorophore labeling of proteins while preserving native functions is essential for bulk Förster resonance energy transfer (FRET) interaction and single molecule imaging analysis. Here we describe a versatile, efficient, specific, irreversible, gentle and low-cost method for labeling proteins with fluorophores that appears substantially more robust than a similar but chemically distinct procedu...

The loss functions of deep neural networks are complex and their geometric properties are not well understood. We show that the optima of these complex loss functions are in fact connected by a simple polygonal chain with only one bend, over which training and test accuracy are nearly constant. We introduce a training procedure to discover these high-accuracy pathways between modes. Inspired by...

conclusions the results suggest that fge can improve the antibiotic sensitivity of these pathogens to some antibiotics. results fresh garlic extract displayed evident inhibition properties against c. albicans and mrsa, yet weak inhibition properties against p. aeruginosa. additionally, fge showed the potential to improve the effect of antibiotics on antibiotic resistant pathogens. the synergism...

in this paper, we use petrov-galerkin elements such as continuous and discontinuous lagrange-type k-0 elements and hermite-type 3-1 elements to find an approximate solution for linear fredholm integro-differential equations on $[0,1]$. also we show the efficiency of this method by some numerical examples

‎in this paper, we discuss about existence of solution forintegro-differential system and then we solve it  by using the petrov-galerkin method. in the petrov-galerkin method choosing the trial and test space is important, so  we use alpert multi-wavelet as basisfunctions for these spaces. orthonormality is one of theproperties of alpert multi-wavelet which helps us to reducecomputations in the...

BACKGROUND Recently, magnetic resonance (MR)-conditional implantable cardioverter defibrillator (ICD) systems have become available. However, associated cardiac MR image (MRI) quality is unknown. The goal was to evaluate the image quality performance of various cardiac MR sequences in a multicenter trial of patients implanted with an MR-conditional ICD system. METHODS AND RESULTS The Evera-MR...

We present a topological characterization for fuzzy regular languages: we show that there is a bijective correspondence between fuzzy regular languages and the set of all clopen fuzzy subsets with finite image in the induced fuzzy topological space of Stone space (Profinite space), and then we give a representation of closed fuzzy subsets in the induced fuzzy topological space via fuzzy regular...

Formylglycine generating enzyme (FGE) performs a critical posttranslational modification of type I sulfatases, converting cysteine within the motif CxPxR to the aldehyde-bearing residue formylglycine (FGly). This concise motif can be installed within heterologous proteins as a genetically encoded "aldehyde tag" for site-specific labeling with aminooxy- or hydrazide-functionalized probes. In thi...