We report the preparation of aminopropyl-functionalized magnesium phyllosilicate (Mg-organoclay) conjugated to fluorescein isothiocyanate (FITC) functionality (FITC-organoclay) by one-pot sol-gel synthesis. The physical characteristics of the Mg- and FITC-organoclays were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), FT-IR and UV-Vis spectrophotometry. X-ray fluoresce...

A novel fluorescence nanoprobe (reduced nano-graphene oxide [nrGO]/fluorescein isothiocyanate-labeled peptide [Pep-FITC]) for ultrasensitive detection of matrix metalloproteinase 2 (MMP2) has been developed by engineering the Pep-FITC comprising the specific MMP2 substrate domain (PLGVR) onto the surface of nrGO particles through non-covalent linkage. The nrGO was obtained by water bathing nano...

Using spectrofluorimetry and fluorescence microscopy, we analyzed the uptake and degradation of fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-albumin) by the rat visceral yolk sac (VYS) during whole embryo culture. Rat conceptuses exposed continuously to FITC-albumin had linear increases of both acid-soluble and acid-insoluble FITC fluorescence in the VYS. Smaller amounts of ...

In this paper, we characterize the uptake mechanism of fluorescein isothiocyanate-labeled human immunoglobulin G (FITC-hIgG) in opossum kidney (OK) epithelial cells, which have been shown to express megalin and cubilin. Confocal immunofluorescence microscopy showed the punctate expression of the neonatal Fc receptor FcRn in the cytoplasm, but not on the cell surface membrane. Temperatureand ene...

PURPOSE To assess the effects of retro-orbital (RO) injection of fluorescein isothiocyanate dextran (FITC-dextran) for observing mouse retinal vessels. METHODS Oxygen-induced retinopathy (OIR) was induced in 7-day postnatal (P7) C57BL/6J mice by exposing them to a 75% oxygen atmosphere for 5 days and then returning them to room air at P12. At P17, 45 P17 OIR mice and 12 normal mice received a...

OBJECTIVE To develop a simple method to purify blastomeres of inner cell mass (ICM) and trophectoderm (TE) lineage using magnetic activated cell sorting. DESIGN Prospective laboratory study. SETTING Embryology research laboratory. PATIENT(S) None. INTERVENTION(S) Trophectoderm cells of zona-free blastocysts were labeled with concanavalin A conjugated to FITC, and every nucleus in the bl...

Novel amine-functionalized symmetrical SQ-dye and FITC-SQ-FITC dye conjugate were designed successfully synthesized, followed by their structural photophysical characterizations. Symmetrical SQ exhibited complete fluorescence quenching in polar solvents, which was explained considering photoinduced electron transfer (PET) as a dominant pathway. On the other hand, resulted decrease an aggregatio...

Organic fluorescence agent designed into nanoparticle can significantly promote its fluorescent efficiency. In this pape, a novel fluorescein isothiocyanate labeling aminated agarose (FITC-AA) is prepared and tested as an effective fluorescent labeling agent. IR analysis and EA proved that FITC was grafted to the aminated agarose (AA) successfully. FITC-AA spontaneously formed nanoparticle with...