conclusions based on our findings, we conclude that the separation and reproduction of adipose tissue cells is an appropriate method for purification of mscs in animal studies. regarding the histomorphometric and flow cytometry analysis results, we demonstrated the differentiation ability of mscs in normal medium and hope to employ these cells for the regeneration of damaged bone tissues in the...

Background: Regarding the strong differentiation and anti-leukemic activity of Dendrostellera lessertii extract, an active agent with similar capabilities was isolated from the crude extract of the plant leaves. The aim of this study was to determine whether the anti-proliferative effect observed for this compound, is differentiation-dependent among the drug-treated cells, using U937 cells. Me...

Background: Conventional anti-cancer treatments, such as surgery, chemotherapy, radiation therapy, etc., are linked to antimicrobial resistance (AMR) and have negative effects on healthy tissues. Objectives: The flow cytometry technique was used in this study to evaluate the effectiveness of a modified anti-cancer peptide in treating the AGS gastric cancer cell line. Methods: This study used ...

background: flow cytometry is a rapid, sensitive, and reliable method for determination of bacterial viability. here we assayed the capability of flow cytometry to detect helicobacter pylori viable cells in both forms of spiral and coccoid. methods: viable bacteria stained with rhodamin 123 and fluoresced with laser beam of 488nm. the rate of rh123 absorption was determined in both forms of bac...

background: human cd34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (hscs) and the small- vessel endothelial cells of a variety of tissues. cd34 plays a critical role as a marker for diagnosis and classification of leukemia. anti cd34 antibodies are used for isolation and purification of hscs from bone marrow, peripheral blood and cord blood. objective: t...

Background: Human CD34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (HSCs) and the small- vessel endothelial cells of a variety of tissues. CD34 plays a critical role as a marker for diagnosis and classification of leukemia. Anti CD34 antibodies are used for isolation and purification of HSCs from bone marrow, peripheral blood and cord blood. Objective: ...

cytotoxicity evaluation of daphne mucronata (thymelaeaceae) extract and one of its active purified components, using seven different cancerous cell lines, revealed the strong antiproliferative activity of the plant. using flow cytometry technique, it was found that treatment of the most responsive cells (k562) with the plant extract or the active component inhibited the progression of cells thr...

brucellosis is an infectious disease and it seems that it affects human immune system and can cause acute, subacute and chronic clinical features. forty patients suffering from chronic brucellosis were studied for cd3+, cd4+,cd8+, cd19+, cells and cd4+/cd8+ ratio by flow cytometry before and after treatment with antibiotics and immunopotentiators. the results were compared with 15 healthy contr...

hepatitis b virus (hbv) is considered as a global health concern and hepatitis b surface antigen (hbsag) is the most immunogenic protein of hbv. the purpose of this study was to evaluate the expression of hbsag on the cell surface of human embryonic kidney cell line (hek293t). after transformation of expression vector pcdna/hbsag to e.coli top10f’, plasmid was extracted and digested with bglii....