A review of the very full literature upon the concentration of ammonia in blood discloses much confusion and divergence in the reported values. For example, the range of values for the systemic blood of normal dogs is from a few hundredths of a mg. (Folin and Denis, 1) to 5 mg. (MacCallum and Voegtlin, 2) or more of ammonia nitrogen in 100 cc. Even more pronounced are variations in the data of ...

In the classical theory of protein metabolism proposed by Folin (1) two types of protein metabolism are recognized, one a constant or endogenous metabolism, and the other a variable or exogenous metabolism. According to this theory the endogenous metabolism is represented by the excretion of creatinine at a relatively constant rate which is independent of protein intake. More recently, as point...

Phenolic compounds were measured in leaf tissues of apple cvs Jonagold and Golden Delicious, healthy and infected by Venturia inaequalis. Leaves were sampled from May to September 2005 and analyzed by high performance liquid chromatography. Hydroxycinnamic acids detected were chlorogenic, caffeic, ferulic and pcoumaric. In addition, the presence was ascertained of the dihydrochalcone phloridzin...

Some of the variables in the Folin method for measurement of urinary creatine and creatinine were studied. The effects of varying the picric acid concentration, volume of solution, and heating time on the conversionof creatine to creatinine were investigated. A study was also made of the results obtained in the chromogenic reaction by varying the concentration of picric acid and sodium hydroxid...

The antioxidant potential of the 99% methanolic extract of leaves of Muntingia calabura was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and total phenolic content was measured by Folin-Ciocalteau (FC) by Singleton and Rossi using Gallic acid and Tannic acid as the calibration standard. Moreover Muntingia calabura leaf extract showed strong reducing power and significant antioxidant a...

The methods most commonly employed for the estimation of blood glucose are dependent upon the ability of glucose to reduce alkaline copper solutions. Reagents containing phosphomolybdic acid are then frequently used to form a blue complex by combination with the reduced copper. All these methods suffer the disadvantage that the final colour fades at such a rate as to imperil its accurate compar...

A method for the colorimetric estimation of manganese by reduction of the phosphomolybdicphosphotungstic acid phenol reagent has been described (Abul-Fadl, 1948). Cobalt was also found to give a similar colour reaction in the presence (but not in the absence) of amino-acids. Conversion of cobalt into the cobaltinitrite did not affect its response to this reaction. As methods for the estimation ...

A rapid and simple spectrophotometric procedure is described for the determination of ajmaline and brucine. The method is based on the development of blue coloured product due to reduction of tungstate and/or molybdate in Folin Ciocalteu’s reagent (FCR) by ajmaline and brucine in alkaline medium. The colour is stable for more than 48 h. The chromogenic reaction has max at 540 nm with molar abso...