background: isolation of high quality genomic dna is one of the most important steps in molecular biology studies related to food borne parasites. usually, various protocols are used for different tissues but so far, there is no common, simple and cost benefit procedure for genomic dna extraction of linguatula serrata ( l. serrata .) larvae as a pentastomida endangering food safety. one of the ...

background: infestation of the skin by the “itch mite” sarcoptes scabiei var. hominis results in a contagious skin infection in humans called “sca-bies”. by resolving morphology issues, the present study was designed to be acquainted with itch mite by molecular markers. methods: the mite samples were collected from scabies patients by visiting government hospitals of twin city, pakistan. for su...

background: extraction of blood genomicdnais one of the main approaches for clinical and molecular biology studies. although several methods have been developed for extraction of blood genomic dna, most of these methods consume long time and use expensive chemicals such as proteinase k and toxic organic solvent such as phenol and chloroform. the objective of this study was to developed easy and...

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

In order to perform breeding programs and improve production of native chickens, preserving genetic diversity in different areas of Iran is important due to the reduced available population. Genome sequencing is considered the most functional approach to determine the phylogeny relation between close populations. The aim of the present study was the evaluation of the phylogeny and genetic nucle...

The nature and pace of genome mutation is largely unknown. Standard methods to investigate DNA-mutation rely on arraying or sequencing DNA from a population of cells, hence the genetic composition of individual cells is lost and de novo mutation in cell(s) is concealed within the bulk signal. We developed methods based on (SNP-) arraying and next-generation sequencing of single-cell whole-genom...

a rapid and sensitive reverse transcription polymerase chain reaction (rt-pcr) and nested-pcr were used to detect bovine viral diarrhea virus 1 (bvdv-1) in bull semen. selected primers could amplify a part of the 5´utr of the bvdv genome. a 294 bp dna fragment was amplified and specificity of the results was confirmed by direct sequencing of the pcr product. prior to rna extraction, the seminal...

colorectal cancer (crc) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. analysis of dna extracted from stool specimens is a recent advantage to cancer diagnostics. many protocols have been recommended for dna extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...

Background: Haplotyping is invaluable not only to identify genetic variants underlying a disease or trait, but also to study evolution and population history as well as meiotic and mitotic recombination processes. Current genome-wide haplotyping methods rely on genomic DNA that is extracted from a large number of cells. Thus far random allele drop out and preferential amplification artifacts of...

objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...