High-molecular weight (HMW (glutenin subunits are encoded by the Glu-1 loci (Glu-A1, Glu-B1 and <sp...

to evaluate allelic diversity at loci encoding low molecular weight glutenin subunits (lmw-gs) among 62 genotypes of bread wheat, nine specific primer pairs for glu-a3, glu-b3, glu-d3 loci were employed. pcr products were separated on 2% agarose gels. two pairs of specific primers, at amplified glu-a3 locus, 8 alleles were detected the c allele of which was the most abundant with a frequency of...

arthrobotrysamerospora (atcc 34468) produced glucoamylase in a semi-synthetic medium containing starch as a sole carbon source. polyacrylamide gel electrophoresis of crude glucoamylase showed three isoenzymes. they were designated as glu i, glu ii and glu iii according to their electrophoretic mobility. these iso-glucoamylases were purified by column chromatography using deae-sephadex a-50. the...

Long-term consumption of rapidly digestible starches is a major factor associated with diabetes and other metabolic diseases. In this study, cassava starch (CS) was used to investigate interactions between different forms glutenin protein the effect these on in vitro digestibility. First, CS natural (Glu), hydrolysate (GPH), or citric acid modified (CA-Glu) were heat treated. The physicochemica...

Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW/LMW-GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern using SDS-PAGE and their PCR based on DNA markers in 60 advanced wheat lines and cultivars...

We propose a gradient-based block coordinate descent (BCD-G) framework to solve the joint approximate diagonalization of matrices defined on product complex Stiefel manifold and special linear group. Instead cyclic fashion, we choose optimization based Riemannian gradient. To update first variable in manifold, use well-known line search method. second group, four different kinds elementary tran...