Theophylline-7-acetic acid (7-TAA) derivatives with several amino acids were screened for antiproliferative effects on acute (HL-60) and chronic (K-562) myeloid leukemia cells as well as on non-malignant cells derived from embryonic human kidney (HEK 293T). The anti-proliferative effects (IC50) were revealed at relatively high concentrations, between 330.4 and 1051.9 μM, but the tested compound...

Kim EY, Choi K-J, Dryer SE. Nephrin binds to the COOH terminus of a large-conductance Ca -activated K channel isoform and regulates its expression on the cell surface. Am J Physiol Renal Physiol 295: F235–F246, 2008. First published May 14, 2008; doi:10.1152/ajprenal.00140.2008.—We carried out a yeast two-hybrid screen to identify proteins that interact with large-conductance Ca -activated K (B...

We carried out a yeast two-hybrid screen to identify proteins that interact with large-conductance Ca2+-activated K+ (BKCa) channels encoded by the Slo1 gene. Nephrin, an essential adhesion and scaffolding molecule expressed in podocytes, emerged in this screen. The Slo1-nephrin interaction was confirmed by coimmunoprecipitation from the brain and kidney, from HEK-293T cells expressing both pro...

Background: Human FIX (hFIX) gene transfer into hepatocytes has provided a novel approach for treatment of hemophilia B. To obtain an improved expression of hFIX, the functional hFIX-expressing plasmids with appropriate intron-derived fragments which facilitate transcription and promote an efficient 3′-end formation of mRNAs are required.Objectives: We ai...

BACKGROUND Biotin is an essential micronutrient and its levels are high in rapidly proliferating cells such as cancer cells. We hypothesized that the synthesis of poly (amido)amine (PAMAM) dendrimers with biotin molecules might contribute to enhanced cancer cell-specific uptake. MATERIALS AND METHODS PAMAM dendrimers were biotinylated using sulfo-NHS-LC-biotin and structural characterization ...