background: the purpose of this study was to investigate the enhanced thermal resistance mechanism of the du145 tumor spheroid cultures as compared to the prostate carcinoma cell line's monolayer cultures. materials and methods: du145 cells were cultured either as spheroids or monolayers. cultures were treated with hyperthermia in a precision water bath (at 43°c for 60 minutes) and/or quercetin...

Induction of heat shock proteins (hsps) is considered as an important protective, ecophysiologically adaptive, and genetically conserved response to environmental stress in all organisms. The effects of exposure to sublethal doses of CdCl2 (0.05, 0.1 and 0.2 LC50) during 14 days on mRNA-hsp70 expression in liver and gillwere investigated in juveniles of Persian sturgeon (Acipenser persicus). Fi...

the present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza a m2-protein (m2e), which was fused to the n-terminus of the truncated hsp70 (hsp70359–610) molecule as a new approach for future vaccine research against influenza a. the amplified fragments, m2e and hsp70359-610 genes, were gel-purified. the products were then single digested with bam...

Background and Aims: Since the produced recombinant proteins by molecular genetics techniques commonly have some limitations in the application, chimeric protein are introduced. Chimeric proteins have found widespread application for the study of protein folding, structure stability, function and immunogenisity. Methods: According to the known Immunomodulatory effect and structure of HSP70 mole...

Induction of heat shock proteins (hsps) is considered as an important protective, ecophysiologically adaptive, and genetically conserved response to environmental stress in all organisms. The effects of exposure to sublethal doses of CdCl2 (0.05, 0.1 and 0.2 LC50) during 14 days on mRNA-hsp70 expression in liver and gillwere investigated in juveniles of Persian sturgeon (Acipenser persicus). Fi...