In this paper we present a system for the detection of immunogold particles and a Transfer Learning (TL) framework for the recognition of these immunogold particles. Immunogold particles are part of a high-magnification method for the selective localization of biological molecules at the subcellular level only visible through Electron Microscopy (EM). The number of immunogold particles in the c...

Extracellular immunoglobulin (IgG) deposits were shown by both light and electron microscopy in renal biopsy material using immunogold labelling. After fixation of tissue in 4% paraformaldehyde and embedding in Lowicryl K4M, semithin sections were cut and stained using the immunogold silver stain. The sections were then viewed and areas of interest were noted; ultrathin sections were cut from t...

The locations of cytochrome cd1 nitrite reductases in Pseudomonas aeruginosa and Pseudomonas fluorescens and copper nitrite reductases in Achromobacter cycloclastes and Achromobacter xylosoxidans were identified. Immunogold labeling with colloidal-gold probes showed that the nitrite reductases were synthesized exclusively in anaerobically grown (denitrifying) cells. Little immunogold label occu...

background: cystic echinococcosis is an important zoonosis caused by the echinococcus granulosus with a substantial impact in human and animal health in en-demic areas. the purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method. methods: eight dogs were challenged with protoscoleces in order to have posi-tive echinococcosis ...

Immunogold labeling is similar to conventional immunofluorescence techniques, generally used for light microscopy. However, immunogold electron microscopy provides investigators with an added dimension over the usual fluorescence assays, as the localization and distribution of macromolecules can be clearly observed at an ultrastructural level, at high magnifications, with high resolution. In ad...

OBJECTIVES The objective of this study was to investigate the silver gull as an indicator of environmental contamination by salmonellae and carbapenemase-producing Enterobacteriaceae (CPE) in south-east Australia. METHODS A total of 504 cloacal samples were collected from gull chicks at three nesting colonies in New South Wales, Australia [White Bay (n = 144), Five Islands (n = 200) and Monta...

Polyclonal antisera have been raised against purified Acanthamoeba myosin II and to a synthetic 26 amino acid peptide that corresponds in sequence to the phosphorylation site of Acanthamoeba myosin IC. These antisera are specific for their respective antigens as determined by immunoblotting after SDS-PAGE of total cell lysates. By using the antisera, localization studies were performed by indir...