In this article a hydrodynamic and rheological analysis of a continuous airlift bioreactor with high-cell-density system is presented. A highly flocculating recombinant strain of Sacharomyces cerevisiae containing genes for lactose transport (lactose permease) and hydrolysis (beta-galactosidase) was exploited to ferment lactose from cheese whey to ethanol. The magnetic particle-tracer method wa...

−A packed bed external loop airlift bioreactor (PBELAB) was proposed as an alternative treatment system for wastewater containing ammonia and nitrate compounds. The 60L PBELAB consisted of aeration and non-aeration zones, both of which were packed with plastic bioballs to enhance the surface area for the attachment of bacteria. The system was able to achieve complete removal of all nitrogen com...

The potential of two zygomycetes fungi, Mucor indicus and Rhizopus oryzae, in assimilating citrus waste free sugars (CWFS) and producing fungal chitosan, oil, and protein as well as ethanol was investigated. Extraction of free sugars from citrus waste can reduce its environmental impact by decreasing the possibility of wild microorganisms growth and formation of bad odors, a typical problem fac...

Continuous fermentations were carried out with a recombinant flocculent Saccharomyces cerevisiae strain in an airlift bioreactor. Once operating under steady state at a dilution rate of 0.45 h(-1), the bioreactor was contaminated with Escherichia coli cells. The faster growing E. coli strain was washed out of the bioreactor and the recombinant, slower growing flocculating S. cerevisiae strain r...

A bioreactor refers to any manufactured or engineered device that supports a biologically active environment. These kinds of reactors are designed to treat wastewater treatment. Volumetric mass transfer coefficient and the effect of superficial gas velocity, as the most important operational factor on hydrodynamics, in three-phase airlift reactors are investigated in this study. The experiments...

In recent years, there has been a growing interest in bioreactors, which utilise immobilised enzymes and cells in order to improve the bioprocess productivity. In fact, there are many specific advantages of the immobilised systems in comparison with the more conventional systems, in which the bio-agents are suspended freely (cells) or dissolved in a bulk aqueous medium (enzymes): • high cell de...

The interfacial area a was measured by the sulfite oxidation method in a three-phase external loop airlift bubble column suspending completely the different concentrations of ion exchange resin particles in aqueous carboxymethyl cellulose (CMC) solutions with a wide range of viscosity. The column had been previously studied for the circulating liquid velocity UL, gas holdup G and volumetric gas...

The aim of this work is to use optimization as a tool for scale up in airlift bioreactors and apply this approach to a real life process. To put this approach into practice, Chlamydomonas reinhardtii, a green microalgae, was used as a model microorganism. The effects of the design parameters on the performance of the bioreactors were monitored through the changes in mixing and circulation times...

Cell Culture Bioreactors Basic Types of Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Stirred Tank (Well Mixed) vs. Tubular Reactor (Plug Flow) . . . . . . . . . . . . . . . . . 3 Segregated Bioreactors (Dead Zone Present) Compartmentalized Bioreactors . . 4 Implication When Growth or Reaction Occurs in the Reactor . . . . . ....