Social & Scientific Systems, Inc., Durham, NC 27713, USA Integrated Laboratory Systems, Inc., Research Triangle Park, NC 27709, USA National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA Procter & Gamble NV, Stro...

The alignment of the 28S gene of several species of Pezizales allowed to select two pairs of primers able to amplify the internal transcribed spacer region of ribosomal DNA in mycorrhizal fungi, such as truffles. The higher yield of the amplification product demonstrates a better annealing of the new primers to the rDNA, as compared to the universal primers internal transcribed spacer 1 and int...

Three dictyostelid isolates were found in Spain and Argentina that are morphologically different from known species. These isolates have some features similar to Dictyostelium sphaerocephalum (Oudem.) Sacc., Marchal & É.J. Marchal, but differ in size and sorocarp branching pattern. We sequenced the nuclear ribosomal internal transcribed spacer region to explore phylogenetic relationships among ...

Functional and ecological signiŽcance of rDNA intergenic spacer variation in a clonal organism under divergent selection for production rate Elena Gorokhova, Thomas E. Dowling, Lawrence J. Weider, Teresa J. Crease and James J. Elser Department of Biology, Arizona State University, Tempe, AZ 85287-1501, USA The University of Oklahoma Biological Station, HC-71, Box 205, Kingston, OK 73439, USA De...

The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has potential provide a powerful tool for accurate and rapid monitoring. Unfortunately, many cases, lack universal standards undermines widespread application metabarcoding. One most important considerations plants, aside from selecting p...

From a rRNA repeating unit (9.1 kbp) of mustard we have The DNA was sequenced according to the method of Maxam sequenced the first internal transcribed spacer, the 5.8S rRNA and Gilbert gene, and the second internal transcribed spacer [1]. Here, we present the nucleotide sequence of a 5500 bp fragment containing the 3 ' end terminus of the 25S gene (486 bp), the intergenic spacer REFERENCES (32...