Enterotoxigenic Escherichia coli (ETEC) infections result in large economic losses in the swine industry worldwide. The organism causes diarrhea by adhering to and colonizing enterocytes in the small intestines. While much progress has been made in understanding the pathogenesis of ETEC, no homologous intestinal epithelial cultures suitable for studying porcine ETEC pathogenesis have been descr...

Mannose-resistant hemagglutination (MRHA) was evaluated for identification of Escherichia coli with K99 fimbriae. The sensitivity and specificity of MRHA, relative to the enzyme-linked immunosorbent assay, were 21 and 79%, respectively. Disagreement between the tests may have been due in part to separation of pili from the cells, with resultant enzyme-linked immunosorbent assay-positive, MRHA-n...

Calf diarrhea due to infection by enterotoxigenic Escherichia coli was treated by administration of glycoprotein glycans derived from bovine plasma. The glycan moieties of the nonimmunoglobulin fraction of plasma mimicked the oligosaccharide moiety of intestinal receptors recognized by K99 pili. These glycoprotein glycans inhibited adhesion of E. coli K99+ ST+ to erythrocyte glycoconjugates in ...

Enterotoxigenic Escherichia coli K99 is one of the dominant pathogens associated with diarrhea of calves. Immunoglobulin Y (IgY), has been used as an inexpensive alternative to antibiotics for the prevention and therapy of several bacterial infections. The study aimed to prepare IgY antibodies against E. coli K99 and to investigate its in vitro effectiveness. E. c...

Pregnant cows and heifers were vaccinated with a crude K99 extract prepared from an Escherichia coli K-12, K99 strain. A similar group, injected with an extract prepared from the K-12 parent strain, served as a control. Eleven calves were born to both groups and challenged orally with a mean of 1.6 x 10(11) bacteria of enterotoxic E. coli B41 (O101:K99+, ST+) at the time of first colostrum upta...

With oral infection of calves by an enterotoxigenic Escherichia coli strain carrying K99, F41, and FY adhesins, colostrums from cows vaccinated against either K99+F41 or FY did not provide protection, but a mixture of the two colostrums did. The association of antibodies directed against the different adhesins is more effective than antibodies directed against one adhesin alone for colostral pr...

To evaluate the effect of widely used parenteral vaccination of dams against neonatal colibacillosis, the virulence factors of the intestinal Escherichia coli flora, namely, O serogroup, enterotoxin(s) produced (heat labile, porcine heat stable, and murine heat stable) and adhesins (K88, K99, and 987P antigens) of 149 piglets from different herds in Sweden were investigated. Three categories we...

Passive protection of neonatal piglets against fatal enteric colibacillosis was achieved with powder preparations of specific antibodies against K88, K99, and 987P fimbrial adhesins of enterotoxigenic Escherichia coli. The antibody powders were obtained by spray drying the water-soluble protein fraction of egg yolks from immunized hens after the lipid components were precipitated with an aqueou...

Twenty-four pregnant cows were vaccinated intramuscularly with K99 extract from enterotoxigenic Escherichia coli and inactivated rotavirus as follows: six cows were injected with 2 ml of oil-adjuvanted vaccine; six cows were injected with 0.5 ml of oil-adjuvanted vaccine; six cows were injected with 4 ml of aluminum hydroxide-adjuvanted vaccine twice with a four-week interval; and six cows were...

Neonatal pigs were inoculated with porcine enterotoxigenic Escherichia coli 431, which carries genes for K99 fimbriae and STaP enterotoxin. Colonies of strain 431 were recovered from feces of pigs for up to 17 days after inoculation and tested for hybridization with gene probes for K99 and STaP. Variants of strain 431 that did not hybridize with the probes were considered to have lost the genes...