The effect of onion and garlic extracts on fungal growth and keratinolytic activity was studied in Trichophyton mentagrophytes as one of the major etiologic agents of human and animal dermatophytosis in Iran and other parts of the world. In order to find out the best keratinase producer for further steps, culture conditions for 30 strains of T. mentagrophytes isolated from human dermatophytosis...

The present study deals with isolation, identification and characterization of feather degrading bacterium. The keratinolytic bacteria were isolated from feather dumped soil. The colonies showed higher keratinase production was identified as Bacillius sp, as per Bergey’s manual method. Keratinase producing Bacillus sp, showed higher enzymes production. The crude filtrate showed specific activit...

An increasing quantity of chickens is being utilized annually in the poultry industry, producing a huge volume of chicken feather waste which presents a high quality supply of keratin. Keratinases possessing high level of keratinolytic activity on insoluble keratin play a crucial role in hydrolyzing chicken feathers. Ever since the discovery of proteolytic ability as well as water solubility of...

Feather keratin is highly resistant to degradation, but some keratinase producing microorganisms can easily degrade these insoluble keratins. These keratinase producing species have an important application in removal of poultry waste and recycled into valuable byproduct. Bacillus sp was screened from soil samples of slaughterhouse and poultry farm area using azokeratin medium. Highest keratina...

Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather ...

The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutations (synonymous codons) according to the codon bias in Pichia pastoris were successfully introduced...

The keratinase from Stenotrophomonas maltophilia (KerSMD) is known for its high activity and pH stability in keratin degradation. However, catalytic efficiency and detergent tolerability need to be improved in order to be used for industrial application. In this work, we obtained several keratinase variants with enhanced catalytic efficiency, thermophilicity, and anti-salt and detergent tolerab...

The effect of onion and garlic extracts on fungal growth and keratinolytic activity was studied in Trichophyton mentagrophytes as one of the major etiologic agents of human and animal dermatophytosis in Iran and other parts of the world. In order to find out the best keratinase producer for further steps, culture conditions for 30 strains of T. mentagrophytes isolated from human dermatophytosis...

Feathers are byproduct waste of poultry processing plant and produced in large amount. A small percentage of feather waste is steamed, chemically treated, ground, to form feather meal a dietary protein supplement for animals. Alternatively, keratin can be biodegraded by some Keratinolytic bacteria and in this study Keratinase producing bacteria and their Keratinolytic enzyme production was inve...

This paper assesses the ability of strains of Aphanoascus fulvescens and Chrysosporium articulatum isolated from soil (phaesol) to degrade native feather keratin. Strains were identified based on phenotypic traits and nucleotide sequencing. Response Surface Methodology was used to optimize cultivation conditions exhibiting the highest keratinolytic activity. The experiments were based on Box-Be...