Keratins are water insoluble proteins of our environment. Being extremely resistant to degradation by proteolytic enzymes, keratins are digested mainly by alkali and keratinase enzymes. The aim of present study was to characterize the indigenous keratinase for potentials of keratin-degrading activities. We report the purification and characterization of keratinase from Bacillus subtilis isolate...

Extracellular keratinase isolated from Aspergillus flavus K-03 was immobilized on calcium alginate. The properties and reaction activities of free and immobilized keratinase with calcium alginate were characterized. The immobilized keratinase showed proteolytic activity against soluble azo-casein and azo-keratin, and insoluble feather keratin. Heat stability and pH tolerance of keratinase were ...

Dermatophyte and yeast species have been screened for their degradative ability towards various keratin substrates. Application of soluble preparation of keratin (KS) of chicken feathers enables a preliminary evaluation of the growth of the fungi and screening of fungal isolates that possesed keratinolytic activity and keratinase enzyme. Five dermatophytes i.e. Trichophyton rubrum, Trichophyton...

This study was focused on purification and characterization of keratinase from Bacillus thuringiensis TS2, isolated from feather dumping soil site. Zymogram of crude enzyme on native-PAGE presented bands with keratinase activity of molecular weights is 41 kDa. In purification steps 2.69 fold purification was achieved after 80% ammonium precipitation of the with 34.42% recovery. In further purif...

Feather keratin is highly resistant to degradation, but some keratinase producing microorganisms can easily degrade these insoluble keratins. These keratinase producing species have an important application in removal of poultry waste and recycled into valuable byproduct. A feather-degrading bacterium with high keratinase activity was isolated and identified as Bacillus megaterium (A1). The sel...

Keratinase producing bacterial strains were isolated from the chicken feather dumping site. Keratinase producing bacterial strains were identified by physical, biochemical characteristics, fatty acid methyl ester (FAME) and 16S rDNA sequences. Medium optimization of the selected keratinase producing bacterial strain (Bacillus thuringiensis TS2) was checked with various parameters such as incuba...

the effect of onion and garlic extracts on fungal growth and keratinolytic activity was studied in trichophyton mentagrophytes as one of the major etiologic agents of human and animal dermatophytosis in iran and other parts of the world. in order to find out the best keratinase producer for further steps, culture conditions for 30 strains of t. mentagrophytes isolated from human dermatophytosis...

background and objectives: dermatophytes possess a wide array of virulence factors and various antifungal susceptibility patterns which influence their pathogenesis in humans and animals. the aim of this study was to evaluate antifungal suscep- tibility and keratinase and proteinase activity of 49 dermatophyte strains from the genera microsporum , trichophyton and epidermophyton which were isol...

A keratinolytic enzyme secreted by Aspergillus flavus K-03 cultured in feather meal basal medium (FMBM) containing 2% (w/v) chicken feather was purified and characterized. Keratinolytic enzyme secretion was the maximal at day 16 of the incubation period at pH 8 and 28℃. No relationship was detected between enzyme yield and increase of fungal biomass. The fraction obtained at 80% ammonium sulfat...

The aim of this study was to find the optimal conditions of a newly isolated Streptomyces strain JRS 19. Screening for keratinase producing bacteria and their keratinase production were investigated. The keratinase produced by the isolate was purified by chromatographic techniques and the optimum pH maintained was 7.0 and maintained at different temperatures Viz., 20ºC, 25ºC, 30ºC, 35ºC and 40º...