background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

patients and methods in this study, 87 specimens from iranian patients were amplified using nested pcr amplification of the ul97 gene. sequence analyses of products were performed for identifying the mutated codons. results the present study show that the most frequent gcv-resistant mutations occurred in codons a594v (26.43%), h520q (18.39%), and m460v (13.79%), consequently occurring at a low ...

introduction: haemophilia a is the most common inherited x-linked recessive bleeding disorder. the severity of the resultant bleeding diathesis depends on the fviii levels associated with the mutation. analysis of carrier state can be made indirectly by dna linkage analysis or directly by identifying the mutation that leads to the disease. the aim of this study was to identification of the caus...

in this study, we report a mutation in cybb gene in a patient with x-cgd (diagnosed on the base of family history, ndt test, dhr 123 assay). mutation in cybb gene was detected using sscp analysis (single-strand conformation polymorphism) followed by sequencing. during screening for mutations in the cybb gene we observed 880 c t in exon 8. this mutation resulted in 290 arg stop. we also observed...

after using 3 different generations of antibodies including human and non-human hyperimmune sera, monoclonal antibodies and chimeric antibodies, more recently a newer approach has been developed in which the antibody genes are cloned directly from a patient peripheral b-lymphocytes and expressed in a host like e. coli. in this study the candida albicans serotype a (nctc 3153) mannan was purifie...

conclusions imp3 immunoreactivity was associated with poor differentiation of tumor yet not associated with tumor size depth of invasion or lymph node involvement. results immunoreactivity pattern of imp3 was cytoplasmic in different clinicopathologic findings. among different clinicopathologic findings, we found a statistical relationship between tumor differentiation and imp3 (p = 0.047); so ...

materials and methods a case-control study was designed on 91 cases with spontaneous hcv infection clearance and 259 patients with persistent hcv infection as the control group. the rs12979860 snp was assessed as the most common ifnl polymorphism by pcr-rflp method. results distribution of rs12979860 cc genotype in the spontaneous clearance group was around two folds of its distribution in chro...

abstract ethanol has a vast consumption around the world. many researches confirmed some adverse effect of this component on human health. in addition, recent studies showed significant alteration in both cellular population, and protein profile of human foreskin fibroblast cell line (hfff2) in the specific dosage of ethanol. here, the role and interaction of some proteins (characterized by sig...