The pyridoxal form of alanine aminotransferase from pig heart catalyzes the a,/? elimination reaction with 3chloro-L-alanine as the substrate to form equimolar amounts of pyruvate, ammonia, and chloride. The maximum rate of the a,/3 elimination reaction was 2.5 pmol/min/mg at pH 7.0 (25”(Z), approximately 0.5% that of the transamination reaction between L-alanine and 2-oxoglutarate. Time-depend...

background and objectives: biofilm formation is an important virulence factor for methicillin-resistant staphylococcus aureus (mrsa). fosfomycin is a borad-spectrum antibiotic with inhibitory effects on biofilm production and β-chloro-l-alanine (β-cla) is an amino acid analog. the aim of this study was to determine effect of the combination of fosfomycin and β-cla on biofilm production by mrsa ...

The molar conductivity data of 1-butyl-3-methylimidazolium salicylate, [BMIm][Sal] as an active pharmaceutical ingredient ionic liquid (API-IL) have been determined in water and aqueous solutions of amino acids, glycine and L-alanine at T= (288.15 to 318.15) K. The molar conductivity data were analyzed by low concentration Chemical Model (lcCM) and limiting molar conductivities ( ), ion associa...

Using UV-vis spectrophotometic method, the formation constants for interaction of Cd2+(aq) andCo2+(aq) ions with L-Alanine were experimentally studied at pH = 4.1 ± 0.01 (50mM of potassiumhydrogen phthalate buffer), ionic strength of 0.1M potassium nitrate and at 5 differenttemperatures 15,20, 25,30 and 35°C. The optical absorption spectra of mixtures containingconsidered cations and L-Alanine ...

The present work reported density, viscosity, speed of sound, electrical conductivity and refractive index data of L-alanine in the aqueous solutions of 1-hexyl-3-methylimidazolium ibuprofenate at T= 298.15 K. Using the measured data, partial molar volume of transfer ( ), partial molar isentropic compressibility of transfer ( ), viscosity B-coefficient of transfer (∆traB), ion association const...

Aminotransferases (ATs) interacting with L-alanine are the least studied bacterial ATs. Whereas AlaT converts pyruvate to L-alanine in a glutamate-dependent reaction, AvtA is able to convert pyruvate to L-alanine in an L-valine-dependent manner. We show here that the wild type of Corynebacterium glutamicum with a deletion of either of the corresponding genes does not exhibit an explicit growth ...

Uptake of L-alanine against a concentration gradient has been shown to occur with isolated brush border membranes from rat small intestine. An alanine transport system, displaying the following characteristics, was shown: (a) L-alanine was taken up and released faster than D-alanine; (b) Na+ as well as Li+ stimulated the uptake of both stereoisomers; (c) the uptake of L- and D-alanine showed sa...