Research on the relationship between mitochondrial membrane potential and fermentation profile is being intensely pursued because of the potential for developing advanced fermentation technologies. In the present study, we isolated naturally occurring strains of yeast from sake mash that produce high levels of malic acid and demonstrate that variations in mitochondrial membrane potential correl...

Background: White button mushroom (Agaricus bisporus) is one of the most sensitive agricultural crops after harvesting and its antioxidant properties tend to decrease during post-harvest storage with the passage of time. OBJECTIVES: The possible role of exogenous application of malic acid as dipping treatment on bioactive components and antioxidant properties of sliced white button mushroom dur...

background: white button mushroom (agaricus bisporus) is one of the most sensitive agricultural crops after harvesting and its antioxidant properties tend to decrease during post-harvest storage with the passage of time. objectives: the possible role of exogenous application of malic acid as dipping treatment on bioactive components and antioxidant properties of sliced white button mushroom dur...

Vacuoles isolated from mesophyll protoplasts of the Crassulacean acid metabolism plant Bryophyllum daigremontianum take up [(14)C]l-malic acid from the medium. The initial rates of uptake show saturation kinetics (apparent K(m) about 1 millimolar). In competition experiments, the uptake rates of l-malic acid are greatly reduced in the presence of a surplus of d-malic and tartronic acid. A numbe...

Evidence indicated an accumulation of l-malic acid in the synthetic growth medium of both lysine auxotrophs and wild-type yeast. Malic acid was isolated from growth experiments and was identified by paper and column chromatography, by comparison of infrared spectra with authentic l-malic acid, as well as enzymatically, as a substrate of malic dehydrogenase. Malic acid was accumulated when gluco...

1. The reversible dissociation of lactic and malic dehydrogenases has been studied in detail and compared with the dissociation properties of triosephosphate dehydrogenase, a-glycerophosphate dehydrogenase, and liver alcohol dehydrogenase. 2. Evidence is presented for the existence of an intermediate form of lactic dehydrogenase with catalytic properties altered during reactivation. 3. The tech...

It is known that there is a separation, i.e., compartmentation of organic acids in young maize tissue (11) and that the organic acid prodtucts (specifically malic acid) of CO, fixation are not in complete equilibriuim with mitochondrial pools (9, 10). Thus, there are at least 2 essentially separate metabolic pools of organic acids. Previous work with corn root tips led tus to postulate a metabo...

Relative synthesis of malic enzyme is stimulated 25-to 100-fold by feeding neonatal ducklings or by incubating embryonic chick hepatocytes in culture with triiodothyronine. Synthesis of the enzyme is almost completely blocked when fed birds are starved or when triiodothyronine-treated hepatocytes in culture are also treated with glucagon. Cytoplasmic poly(A)+ RNA was isolated from livers of int...

A mutant malic enzyme gene, mae2-, was cloned from a strain of Schizosaccharomyces pombe that displayed almost no malic enzyme activity. Sequence analysis revealed only one codon-altering mutation, a guanine to adenine at nucleotide 1331, changing the glycine residue at position 444 to an aspartate residue. Gly-444 is located in Region H, previously identified as one of eight highly conserved r...