to investigate the effects of different hormonal treatments on the oocyte maturation in wild- caught grey mullet ( mugil cephalus ), fish were subjected to 4 different hormonal treatments as follows: (a) a control group received 0.5 ml/kg b.w. physiological saline, (b) fish injected with 20 mg/kg carp pituitary extract (cpe) as a priming dose followed by 200 µg/kg sgnrha as resolving dose, (c) ...

background: linoleic acid (la) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence.objective: in the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovinematerials and methods: the experime...

Background In vitro maturation (IVM) of human oocytes has acquired increasing attention in infertility treatment with great promise. This technique is an alternative conventional in vitro fertilization-embryo transfer (IVF-ET), and can be reduced the side effects of gonadotropin stimulation such as ovarian hyperstimulation (OHSS). Oocyte maturation is a complex process including cytoplasmic and...

developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. to accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and fsh concentrations. six-week-old preantral follicles (95 ± 5 µm) were cultured...

In Vitro Maturation (IVM) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. This review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. The review is based on literature reports and the author’s experience. In IVM cycles,...

Background: Successful in vitro maturation (IVM) of oocytes is an important step for production of livestock through assisted reproductive techniques including somatic cell nuclear transfer (SCNT). Obtaining an optimal duration for maturation of oocyte is essential, since over exposure to maturation medium results in oocyte aging, while under exposure leads to production of immature incompetent...

Caspian kutum fish (Rutilus frisii) wild broodstocks were caught by gillnet during their upstream migration and subjected to short-time confinement stress and their physiological responses have been examined. Thirty fish (average weight of 1217.6 g) were divided into three experimental groups CO (control), OV (ovaprim) and CO+OV (cortisol+ovaprim) and placed into rearing tanks (10 fish tank-1; ...