نتایج جستجو برای: melting curve analysis mca

تعداد نتایج: 2935542  

Journal: :Clinical chemistry and laboratory medicine 2008
Daisuke Sasaki Kazuyuki Sugahara Naoko Inokuchi Katunori Yanagihara Hiroo Hasegawa Sayaka Mori Yasuaki Yamada Shimeru Kamihira

BACKGROUND Although mutations in the interferon (IFN) sensitivity determining region (ISDR) of hepatitis C virus (HCV) have been reported to be useful as a predictive viral factor for IFN therapy in patients infected with HCV-1b, such laboratory research has not been favorably translated into the clinic. To promote such translation, we attempted the establishment of a rapid and simple polymeras...

Journal: :Electrochemistry Communications 2010

Journal: :Lab on a chip 2016
Tianlan Chen Yanwei Jia Cheng Dong Jie Gao Pui-In Mak Rui P Martins

We developed a thermal digital microfluidic (T-DMF) device enabling ultrafast DNA melting curve analysis (MCA). Within 7 seconds, the T-DMF device succeeded in differentiating a melting point difference down to 1.6 °C with a variation of 0.3 °C in a tiny droplet sample (1.2 μL), which was 300 times faster and with 20 times less sample spending than the standard MCA (35 minutes, 25 μL) run in a ...

Journal: :Clinical chemistry 2007
Christina Dahl Karen Grønskov Lars A Larsen Per Guldberg Karen Brøndum-Nielsen

BACKGROUND Fragile X syndrome is caused by the expansion of a CGG trinucleotide repeat at the 5' untranslated region of the fragile X mental retardation 1 gene (FMR1). When expanded to >200 repeats (full mutation), the repeat region and the adjacent promoter CpG island become hypermethylated, rendering FMR1 transcriptionally inactive. Conventional molecular diagnosis of fragile X syndrome invol...

2006
Jochen Hurlebaus

480 PCR system Roche Applied Science ́s LightCycler® family of real-time PCR systems offer fast, accurate and versatile platforms for genetic variation research. The new plate-based LightCycler® 480 System provides the temperature homogeneity and optical characteristics required for high-performance melting-curve analysis (MCA). On the level of data acquisition and available detection channels, ...

Journal: :Lab on a chip 2013
Anna Ohlander Caterina Zilio Tobias Hammerle Sergey Zelenin Gerhard Klink Marcella Chiari Karlheinz Bock Aman Russom

The recent technological advances in micro/nanotechnology present new opportunities to combine microfluidics with microarray technology for the development of small, sensitive, single-use, point-of-care molecular diagnostic devices. As such, the integration of microarray and plastic microfluidic systems is an attractive low-cost alternative to glass based microarray systems. This paper presents...

2017
Yiwen Yu Yihui Yao Qinyun Weng Jingyi Li Jianwei Huang Yiqun Liao Fu Zhu Qifeng Zhao Xu Shen Jianjun Niu

Staphylococcus aureus is a global epidemic pathogen that causes heavy disease burden. The aim of this study was to determine which globally known S. aureus lineages are currently present in a hospital of Xiamen. Therefore, the 426 S. aureus strains were detected by Melting Curve Analysis (MCA) and genotyped by Pulsed Field Gel Electrophoresis (PFGE) as well as Multicolor Melting Curve Analysis-...

Letter to editor " Applying High-quality DNA Melting Curve Analysis in Identifying Staphylococcus aureus and Methicillin-resistant Strains "   Ramezan Ali Ataee   Professor, Department of Medical Microbiology, Faculty of Medicine, Hospital Research Development Committee, Applied Microbiology Research Center, System Biology, Poisoning Institute, Baqiyatallah University of Medical Sciences, T...

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