The QuikChange site-directed mutagenesis methodology was applied to constructing a randomly mutagenized plasmid library simply by adding manganese to the reaction mixture. This method is superior to the normally employed Pol I-type polymerase-based error-prone PCR in that (i) it does not require a subsequent ligation reaction, and (ii) there is no accumulation of mutations at the same site. alp...

a pseudomonas aeruginosa mutant derived by random mutagenesis with n-methyl-n¢-nitro-n-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on siegmund-wagner (sw) plates. the mutant designated p. aeruginosa ptcc1637 produces rhamnolipids at concentrations 10 time more than parent strain. nmr analysis and surface tension measurement showed that the biosurfactan...

Post-translational modifications of proteins are ubiquitous in living organisms, as they enable an accurate control the interactions these macromolecules. For mechanistic studies, it would be highly advantageous to able produce vitro post-translationally modified with site-specificity. Here, we demonstrate one facile way achieve this goal through use post-translational chemical mutagenesis. We ...

background: finding shows that there were several problems in the treatment of helicobacter pylori infection such as the emergence of resistance to the antibiotics, the risk of recrudescence, and the high cost of treatment. the ineffectiveness of conventional treatment mechanisms against cancer cells reveals the importance of peptides as a novel therapeutic approach. however, the short length o...

A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N¢-nitro-N-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on Siegmund-Wagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactan...

Introduction Galactose oxidase (GOase; EC 1.1.3.9) is a coppercontaining enzyme that is secreted by certain filamentous fungi, and is the subject of a recent review [ 11. The most extensively studied source of the enzyme is the strain no. 2003 of the Northern Regional Research Laboratory (NKKI,) collection, Peoria, II,, lJ.S.A. [2]. This fungus is designated as Dactylium dendroides [ 3 1, howev...

MOTIVATION Despite many successes of conventional DNA sequencing methods, some DNAs remain difficult or impossible to sequence. Unsequenceable regions occur in the genomes of many biologically important organisms, including the human genome. Such regions range in length from tens to millions of bases, and may contain valuable information such as the sequences of important genes. The authors hav...