DNA double strand breaks (DSBs) are abrasions caused in both strands of the DNA duplex following exposure to both exogenous and endogenous conditions. Such abrasions have deleterious effect in cells leading to genome rearrangements and cell death. A number of repair systems including homologous recombination (HR) and non-homologous end-joining (NHEJ) have been evolved to minimize the fatal effe...

Non-coding RNA sequences outnumber the protein-coding genes in the human genome, however our knowledge of their functions is still limited. RNA-binding proteins follow the transcripts, including non-coding RNAs, throughout their life, regulating not only maturation, nuclear export, stability and eventually translation, but also RNA functions. Therefore, development of sophisticated methods to s...

Crohn disease (CD) is a type of inflammatory bowel that causes inflammation in the digestive tract. Cases CD are increasing worldwide, calling for more research to elucidate pathogenesis CD. For this purpose, usage RNA-sequencing (RNA-seq) technique increasingly appreciated, as it captures RNA expression patterns at particular time point high-throughput manner. Although many RNA-seq datasets ge...

Background and Objective: Extracellular RNA (exRNA) is an evolving concept. In one hand, more endogenous types are detected in various biofluids or found local extracellular space. These exRNAs include microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular (circRNAs), piwi-interacting (piRNAs), transfer (tRNAs) protein-coding messenger (mRNAs). ExRNA profilings associated with many physio...

To explore the influence of long-non coding RNA-H19/microRNA-140-5p/Wnt1 axis on osteogenic differentiation in bone marrow mesenchymal stem cells. First, we used dual-luciferase reporter gene detection to verify coding-H19/miRNA-140-5p/Wnt1 regulatory targeting relationship. Then coding-H19 was overexpressed or silenced cells and then coding-H19, microRNA-140-5p Wnt1 expression were assessed us...

Background:Long non-coding RNAs, as a big part of non-coding RNAs, are considered functionally more than past. These transcripts could be involved in carcinogenesis. SNHG6, as a long non-coding RNA, has been reported to be expressed more in colorectal cancer tissues than non-cancerous ones.  Colorectal cancer as a malignancy needs fast prognostic and diagnostic methods for well...

the genome of iranian wheat stripe virus (iwsv), a tentative member of the genus tenuivirus, is comprised of three ambisense and one negative sense rna segments. the coat and non-structural proteins encoded by the vcrna3 and vrna4 genes, respectively, were efficiently translated in vitro. translated proteins of vcrna3 and vrna4 transcripts were approximately 35000 and 22000 in mr, respectively,...