antibodies directed against the enzyme glutamic acid decarboxylase (gad) are believed to be the main cause of destruction of pancreatic islet cells in type i (insulin dependent) diabetes mellitus. the enzyme was found both in the brain and pancreatic beta cells. although similarities in identity of gad in human and rat brain have been demonstrated, little is known about the interaction between ...

Three flavonoid glycosides including orientin, vitexin, quercetin-3-O-neohesperidoside and one unknown compound were isolated and purified by high-speed counter-current chromatography (HSCCC) and semi-preparative HPLC from Trollius ledebouri Reichb., a traditional Chinese medicine. Preparative HSCCC with a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v) was suc...

Dihydroxyacetone-phosphate:acyl coenzyme A acyltransferase (EC 2.3.1.42) was solubilized and partially purified from guinea pig liver crude peroxisomal fraction. The peroxisomal membrane was isolated after osmotic shock treatment and the bound dihydroxyacetone-phosphate acyltransferase was solubilized by treatment with a mixture of KCl-sodium cholate. The solubilized enzyme was partially purifi...

A function of the eye lens is to absorb near-ultraviolet light (near-UV) transmitted through the cornea and aqueous humor in the wavelength range of about 300-400 nm [1-3], thereby preventing UV-induced photo-damage to the retina [4,5] and reducing chromatic aberration [3]. It has been reported that the majority of environmental UV radiation reaching the lens is UV-A (320-400 nm) and that only ...

Approximately 90% of the total tyrosine hydroxylase activity of bovine adrenal medulla homogenates was localized in the particulate fraction of the homogenate. The particulate enzyme was solubilized by incubation with trypsin, and the solubilized enzyme was partially purified. This enzyme was found to behave like the tyrosine hydroxylase which had been purified from the supernatant fraction by ...

The treatment of tuberculous meningitis is frequently hindered by the formation of exudate within the cerebrospinal spaces. This exudate may obstruct the cerebrospinal circulation and give rise to the complications of subtentorial, basal cistern, and spinal block. Furthermore, the presence of exudate predisposes to the occurrence of tuberculous arteritis, which is responsible for the cerebral a...

Starvation of rats for 48 h increased the activity of PDH (pyruvate dehydrogenase) kinase 2.2-fold in extracts of liver mitochondria, 2.9-fold in PDH complex partially purified therefrom by fractional precipitation, and 5-fold in PDH complex partially purified by gel filtration on Sephacryl S-300. A protein fraction was separated from PDH complex in extracts of rat liver mitochondria by gel fil...

Immunological tolerance to a soluble, partially purified fraction of histocompatibility (H)-2(a) antigen was induced in B10.D2 strain mice. The fraction used was obtained after chromatography on a Sephadex G-150 column. Tolerance of humoral-antibody formation (cytotoxins and hemagglutinins) was observed in mice injected with soluble antigen since birth, but cell-mediated immunity, as measured b...