The protective effect of a L-type calcium channel blocker, nimodipine, on cell injury induced by oxygen-glucose deprivation (OGD) in PC12 cells was investigated. PC12 cells were exposed to in-vitro oxygen-glucose deprivation (30 minutes and 60 minutes respectively) in the presence or absence of nimodipine (10mM/L) in three different time schedules (pre-24h, pre-3h and concurrently). Cellular vi...

background: considering that cannabinoids protect neurons against neurodegeneration, in this study, the neuroprotective effect of win55,212-2 in paraoxon induced neurotoxicity in pc12 cells and the role of the n-methyl-d-aspartate (nmda) receptor were evaluated. materials and methods: in this study pc12 cells were maintained in dulbecco's modified eagle’s medium (dmem+f12) culture medium supple...

micrornas (mirnas) are a class of small non coding regulatory rnas that have key functions in multiple cell processes. deregulation of these tiny mirnas are involved in various human diseases. mir-155 is one of the multifunctional mirna that its over-expression has been found to be associated with different kinds of cancer such as leukemia, breast and colon cancers. it is thought that deregulat...

background and objective: in neurodegenerative disorders, oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages that lead to apoptosis. pro-apoptotic and anti-apoptotic gene expressions were changed during cell differentiation that affect cell viability and differentiation. this study was conducted to determine the effects of hydrogen peroxi...

nowadays, growing interest in the application of nanotechnology in biomedical and biotechnological fields has opened a new avenue to explore the nanoparticles-biological system interaction. indeed, a clear gap is still in the cytotoxic effect of nps on the biological systems. for this purpose, the interaction of the silica oxide nanoparticles (sio2-np) with pc12 cell line, as a model of nervous...

Introduction: The Serum/Glucose deprivation -induced cell death in cultured PC12 cells represents a useful in vitro model for the study of brain ischemia and neurodegenerative disorders. Nigella sativa L. has been known as a source of antioxidants. To elucidate the neuroprotective actions of N. sativa extract in vitro, we studied the effect of N. sativa extract on cultured PC12 cells under s...

Objective(s):Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. Materials and Methods:We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. Results:FA increased cell viability, prevented membrane damage (LDH r...

Application of nanotechnology in medicinal and biological fields has attracted a great interest in the recent yeras. In this paper the cell membrane leakage induced by iron nanoparticles (Fe-NP) against PC12 cell line which is known as a model of nervous system cell line was investigated by the lactate dehydrogenase (LDH) test. Therefore, PC12 cells were incubated with different concentration o...

Background and Objective: In neurodegenerative disorders, oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages that lead to apoptosis. Pro-apoptotic and anti-apoptotic gene expressions were changed during cell differentiation that affect cell viability and differentiation. This study was conducted to determine the effects of hydrogen peroxi...