نتایج جستجو برای: pet22b expression vector

تعداد نتایج: 1048484  

M. H. Sekhavati, M. Naghavi M. R. Nassiri M. Tahmoorespur

Brucellosis is a zoonotic disease in human and animals. Brucella melitensis is one of the most pathogenic species of Brucella in goat and sheep. Omp31 is an outer membrane protein of Brucella that acts as an immunogenic protein. Cytokines are glycoproteins with low molecular weight that play the role of an immune adjuvant and regulate immune responses. Interleukin-2 is one of the most important...

Journal: :Microorganisms 2023

Glycolate oxidase is a peroxisomal flavoprotein catalyzing the oxidation of glycolate to glyoxylate and plays crucial metabolic roles in green algae, plants, animals. It could serve as biocatalyst for enzymatic production glyoxylate, fine chemical with wide variety applications perfumery, flavor, pharmaceutical agrochemical industries. However, low catalytic activity native levels active enzyme...

Journal: :modares journal of medical sciences: pathobiology 2014
shakiba darvish alipour astaneh iraj rasooli seyed latif mousavi gargari

objective: acinetobacter baumannii (a. baumannii) has a good potential to colonize on various surfaces. as a virulence factor, adhesion to surfaces is the first step in colonization. the two-partner secretion system (tps) proteins are key factors for bacterial attachment. the purpose of this study is to identify and study the role of this family of proteins in adhesion of a. baumannii to human ...

Journal: :jundishapur journal of microbiology 0
ava behrouzi department of biology, school of basic science, science and research branch, islamic azad university, tehran, ir iran saeid bouzari department of molecular biology, pasteur institute of iran, tehran, ir iran seyed davar siadat department of tuberculosis and pulmonary research, pasteur institute of iran, tehran, ir iran; department of tuberculosis and pulmonary research, pasteur institute of iran, tehran, ir iran. tel: +98-9121442137, fax: +98-2166492619 anis jafari department of molecular biology, pasteur institute of iran, tehran, ir iran shiva irani department of biology, school of basic science, science and research branch, islamic azad university, tehran, ir iran

conclusions our results showed that the recombinant protein produced by the pbad vector in the escherichia coli system was very efficient. background nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. in...

Asghar Tanomand, Jafar Majidi, Safar Farajnia, Shahin Najar,

Background: Infections due to Pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. At present, aggressive antibiotic therapy is the only choice for management of P. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alt...

Journal: :iranian journal of biotechnology 2013
asghar tanomand shahin najar peerayeh safar farajnia jafar majidi

background: antibiotic resistance and the need for long-term treatments especially for chronic infections necessitate the developmentof a vaccine against pseudomonas aeruginosa infection.objectives: in this study, recombinant exotoxin a (domains i and ii), (exoa i-ii) protein was expressed, purifid and its immunologicalcharacteristics were evaluated in a mouse model.materials and methods: the g...

Background: Conventional anti-cancer treatments, such as surgery, chemotherapy, radiation therapy, etc., are linked to antimicrobial resistance (AMR) and have negative effects on healthy tissues. Objectives: The flow cytometry technique was used in this study to evaluate the effectiveness of a modified anti-cancer peptide in treating the AGS gastric cancer cell line. Methods: This study used ...

Journal: :modares journal of medical sciences: pathobiology 2011
mohammad shenagari farzaneh sabahi masoumah tavasoti kheiri kambiz forghan parast abbas jamali

objective: in this study, two conserved genes (m1 and np) of influenza virus were expressed in a bicistronic vector in order to develop a universal gene based vaccine. materials and methods: plasmids m1-pires2-egfp, pires2-np were constructed by cloning the pcr products of m1 and np genes which were amplified from the a/peurto rico/8/34 (h1n1) influenza virus strain into the plasmid expression...

Journal: :iranian journal of public health 0
hadi mirahmadi 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . ; 2. dept. of parasitology and mycology, zahedan university of medical sciences , zahedan, iran. shirzad fallahi 3. dept. of parasitology and mycology, lorestan university of medical sciences , khoramabad, iran. vahid fallah omrani 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . bahram kazemi 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . ali haghighi 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . seyyed javad seyyed tabaei 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran .

detection of plasmodium vivax specific antibodies with serological tests could be a valuable tool for epidemiological researches. whereas p. vivax cannot be simply obtained in vitro, serological tests using total or semi-purified antigens are infrequently used. given this restriction, the present study investigated whether recombinant p. vivax merozoite surface protein 1 (pvmsp-1 42 kda) could ...

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