Scape bending disorder is the most important factor affecting postharvest loss of gerbera cut flowers. One of the ultimate reasons for gerbera stem bending is lignin, with deformation structural functions and defensive mechanisms. This postharvest experiment was conducted to evaluate the role of phenylalanine ammonia lyase (PAL) and peroxidase (POD) enzymes activity in stem bending of two gerbe...

Salinity is an important environmental factor that limits plant growth and production. Grape is classified as salt sensitive plants. The object of this study was to evaluate effects of salinity on membrane lipid peroxidation, antioxidant components, and antioxidative enzymes activity in ten grape genotypes native to the regions around Urmia Salt Lake.Malondialdehyde content and protective enzym...

A cDNA clone coding phenylalanine ammonia-lyase (PAL) was isolated from a cDNA library prepared from asparagus spears (Asparagus officinalis L. cv. Welcome) using the reverse transcription-polymerase chain reaction (RT-PCR). The partial cDNA clone encoded an mRNA of 527 bp and the derived amino acid sequence was found highly homologous to PAL from rice, maize and barley. Northern blot analysis ...

l-Phenylalanine ammonia-lyase (PAL) activity is low in the external layers (flavedo) of intact mature grapefruit peel. Flavedo discs evince upon incubation increasing PAL activity and ethylene production. Light has no effect in enhancing PAL activity in discs. Exogenous ethylene stimulates PAL activity in the flavedo of intact mature grapefruits (half maximum stimulation at 15 ppm); such activi...

Flavonoids are valuable natural products derived from the phenylpropanoid pathway. The objective of this study was to create a host for the biosynthesis of naringenin, the central precursor of many flavonoids. This was accomplished by introducing the phenylpropanoid pathway with the genes for phenylalanine ammonia lyase (PAL) from Rhodosporidium toruloides, 4-coumarate:coenzyme A (CoA) ligase (...

Plant growth and activity of peroxidases (POX) and phenylalanine ammonia-lyase (PAL) were investigated in seedlings of yellow lupine (Lupinus luteus L.) and soybean (Glycine max L.) treated with copper (25 mg/l) and an inhibitor of ethylene biosynthesis (CoCl 2 ). Both lupine and soybean plants stressed with an excess of copper exhibited a decrease in root length and fresh weight. In lupine pla...

L-Phenylalanine ammonia-lyase has been purified from elicitortreated alfalfa (Medicago sativa L.) cell suspension cultures using two protocols based on different sequences of chromatofocusing and hydrophobic interaction chromatography. Three distinct forms of the intact enzyme were separated on the basis of affinity for Octyl-Sepharose, with isoelectric points in the range pH 5.1 to 5.4. The na...

Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar 'Ataulfo' in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme activity of PAL and C3H was determined in mango fruits during ripening. The PAL ...

The transcript levels of six defense genes including pathogenesis-related gene 1 (PR-1), pathogenesis-related gene 2 (PR-2), pathogenesis-related gene 5 (PR-5), lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL) and catalase (CAT) were investigated in tomato plants inoculated with Xanthomonas axonopodis pv. phaseoli as a non-host pathogen and X. euvesicatoria as a host pathogen. Activation o...

salicylic acid (sa) and methyl jasmonate (meja) as phytohormone regulate synthesis of secondary metabolites in a wide range of plant species thereby accumulation of phenylalanine ammonia lyase enzyme (pal) and accelerate of oxygen free radicals, protein kinas and no production. the present research designed to evaluate effect of sa and meja elicitation on accumulation of phenolic compounds, fla...