the domesticated silkworm, bombyx mori linn., a lepidopteran molecular model and an important economic insect that are emerging as an ideal molecular genetic resource for solving a broad range of biological problems. the silkworm, b. mori produces massive amount of silk proteins during the final stage of larval development. these proteins are stored in the middle silk gland and they are dischar...

morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of b. thuringiensis ssp. kumamotoensis dsm 6070 (bt 6070) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the activity of the spore-crystal suspensions against house fly (musca domestica) larvae wa...

we have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-de). this modified version of 2-de is not only simpler and easier but also faster than all the currently available methods. in this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-...

the indian riverine buffaloes are more susceptible to variations in the environment. these variations affect seminal plasma proteins, which in turn affect fertility. this study was conducted to evaluate variations in the indian bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2d-page) during winter and summer seasons, and its relation with...

Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN) have been compiled and characterized. Usefulness and applicability of the systems for clinical investigations are discussed and illustrated by...

Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel is relatively weak, and one can separate the two forms of apoB in a slab gel apparatus only if on...