background: the effects of extremely low frequency electromagnetic fields (elf-emf) on toxoplasma gondii have not been explained yet. the aim of this study was to assess the possible effects of elf-emf on growth, survival time and viability of toxoplasma gondii . in addition, the life span of toxoplasma infected animals was investigated. methods: sixty adult male balb/c mice were used for in vi...

conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

background and objectives: making stacking gels for polyacrylamide gels in the laboratory by conventional methods is laborious and time consuming. considering the role of temperature in polyacrylamide gels with respect to electrical resistance and viscosity, we assumed that decreasing the temperature would cause an increase in electrical resistance and viscosity.  ultimately, a downward tempera...

SUMMARY Tumor-specific transplantation antigen (TSTA) was solu-bilized from cell membranes of sarcoma Meth-A with non-ionic detergent Nonidet P40. Soluble TSTA was partially characterized by Chromatographie separation and electro-phoresis. The antigen responsible for tumor rejection activ ity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophore...

Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...

MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...

Enzyme with deamidase activity was found in the germinating wheat seeds. The activity appeared in the seeds after 24 hours of germination and reached its maximum value after 48 hours and then declined rapidly. Protein deamidase from germinating wheat seeds was further purified by salting out with phosphate salts and by subsequent chromatography on gel filtration and DEAE-cellulose column chroma...

introduction when a liquid is irradiated with high-intensity and low-frequency ultrasound, acoustic cavitation occurs and there are some methods to determine and quantify this phenomenon. the existing methods for performing these experiments include sonochemiluminescence (scl) and chemical dosimetric methods. the particles in a liquid decrease the ultrasonic intensity threshold needed for cavit...