Methods were developed for the polyacrylamide gel electrophoretic analysis of capsular polysaccharides of bacteria with Escherichia coli K1 as a model. Conditions were determined for the rapid and gentle extraction of the K1 polysaccharide by incubation of the bacteria in a volatile buffer and for the subsequent removal of the putative phospholipid moiety attached to the reducing end of the pol...

Polysaccharide-rich plant cell walls are hydrated under functional conditions, but the molecular interactions between water and polysaccharides in the wall have not been investigated. In this work, we employ polarization transfer solid-state NMR techniques to study the hydration of primary-wall polysaccharides of the model plant, Arabidopsis thaliana. By transferring water (1)H polarization to ...

An efficient synthetic strategy has been developed for the synthesis of a tetrasaccharide related to O-specific polysaccharide from Escherichia coli O132 as their 2-aminoethyl glycosides in very good yield by adopting sequential glycosylation followed in-situ removal p-methoxybenzyl (PMB) group same reaction pot. Furthermore, route was adapted carrying out three stereoselective iterative glycos...

NMR spectroscopy can be used to characterize bacterial polysaccharides such as that of Streptococcus pneumoniae type 1 which is a component of the 23-valent pneumococcal vaccine in clinical use. This particular polysaccharide gives NMR spectra with wide lines apparently due to restricted molecular mobility and chain flexibility which leads to rapid dipolar T(2) relaxation limiting the possibili...

Polysaccharide antigens of Pasteurella multocida serotype B:2,5 were identified by immunodiffusion and immunoelectrophoresis. The immunodiffusion tests showed that the first line, near the well containing the whole polysaccharide extract (WPE) represent LPS because this precipitation line occurs by diffusion using antiserum raised against P. multocida serotypes 8:2,5 and E:2,5 but not with 8:3,...

In the present study, properties of silver nanoparticles (AgNPs) such as average size, size distribution andmorphology were investigated by Tollens, polysaccharide, modified polysaccharide and microbial methods.The synthesized AgNPs were characterized by UV-visible spectroscopy, scanning electron microscopy(SEM), transmission electron microscopy (TEM), dynamics light scatterin...

A crude polysaccharide-protein complex from Lactarius deliciosus fruit bodies (LDP) was extracted with hot water, precipitated, and washed with 95% ethanol. After protein removal according to the Sevag method, LDP was dialyzed against running tap water and distilled water, and further purified by Sephadex G-100 column chromatography. Gas chromatography showed that LDP is composed of glucose, ga...

Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPL...