Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...

Monoclonal and oligoclonal banding has been observed in electrophoretograms of serum, cerebrospinal fluid, and urinary protein from patients infected with the human immunodeficiency virus (HIV). This is the first report of kappa oligoclonal banding in the protein electrophoretograms for urine but not for serum of two patients with the acquired immune deficiency syndrome (AIDS). Both patients ha...

The present study evaluated the impact of moderate exercise training on the cardiac tolerance to acute pressure overload. Male Wistar rats were randomly submitted to exercise training or sedentary lifestyle for 14 wk. At the end of this period, the animals were anaesthetized, mechanically ventilated, and submitted to hemodynamic evaluation with biventricular tip pressure manometers. Acute press...

Growth hormone (GH) is the most important hormone that controls somatic cell growth and the syntheses of protein, fat, and carbohydrates. This study was to identify gene polymorphism of GH-1 using PCR- single strand conformation polymorphism (SSCP) technique and its association with growth traits including condition factor (CF), body weight (BW) and total length (TL) in common carp (Cyprinus ca...

Right ventricular (RV) and left ventricular (LV) myocardium differ in their pathophysiological response to pressure-overload hypertrophy. In this report we use microarray and proteomic analyses to identify pathways modulated by LV-aortic banding (AOB) and RV-pulmonary artery banding (PAB) in the immature heart. Newborn New Zealand White rabbits underwent banding of the descending thoracic aorta...

Growth hormone (GH) is the most important hormone that controls somatic cell growth and syntheses of protein, fat, and carbohydrates. This study was conducted to identify gene polymorphism of GH-1 using PCR -single strand conformation polymorphism (SSCP) technique and its association with growth traits including condition factor (CF), body weight (BW) and total length (TL) in common carp (Cypr...

The structure of harlequin-stained chromosomes following substitution with low levels of 5-bromodeoxyuridine (BrdUrd) over two cell cycles and high levels over the last part of one cycle (replication banding) was studied in Chinese hamster ovary (CHO) cells. By using correlative light (LM) and scanning electron microscopy (SEM), it was shown that the effects of both the ultraviolet light (u.v.)...

On the basis of the role of small, leucine-rich proteoglycans (SLRPs) in fibrogenesis and inflammation, we hypothesized that they could be involved in cardiac remodeling and reverse remodeling as occurs during aortic stenosis and after aortic valve replacement. Thus, in a well-characterized aortic banding-debanding mouse model, we examined the SLRPs decorin and lumican and enzymes responsible f...

Soluble proteins were extracted from seed and seedlings of eleven oats cultivars to analyze the protein profile through SDS PAGE method. In seed protein electrophoresis totally 14 bands were observed, in these six bands were common to all cultivars. Based on the seed protein banding pattern two of these cultivars were differentiated easily. The unique band at Rf value 0.151 was common for all c...