in the near future, design of a new generation of drugs targeting proteins will be required. considering the complex bond between the drug and protein, the structure and stability of the target protein should be considered. so far, a series of in vitro investigations have been conducted with the aim of predicting drug-biological medium interactions. in these studies, use of spectroscopic method...

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a robust and powerful tool for studying biomacromolecules their interactions. However, quantitative detection of high-mass analytes (kDa to MDa range) remains challenging MALDI-MS. Herein, we successfully used commercially available purified proteins (?-galactosidase BSA) as internal standards MALDI-MS analysis achieved...

Elucidation of Protein-Protein Interactions (PPIs) in living cells/animals is very important for the study basic biology and development novel therapeutics. Recent advances techniques profiling PPIs, such as fluorescence microscopy various proximity labeling-based methods coupled with mass spectrometry (MS), have provided a great opportunity to PPIs their native states spatiotemporal resolution...

Proteins carry out their function in a cell through interactions with other proteins. A large scale Protein-Protein Interaction (PPI) network of an organism provides static yet an essential structure of interactions, which is valuable clue for understanding the functions of proteins and pathways. PPIs are determined primarily by experimental methods; however, computational PPI prediction method...

Knowledge of protein-protein interactions (PPIs) is important for identifying the functions of proteins and the processes they are involved in. Although data of human PPIs are easily accessible through several public databases, these databases do not specify the human tissues in which these PPIs take place. The TissueNet database of human tissue PPIs (http://netbio.bgu.ac.il/tissuenet/) associa...

Druggable Protein-protein Interaction Assessment System (Dr. PIAS) is a database of druggable protein-protein interactions (PPIs) predicted by our support vector machine (SVM)-based method. Since the first publication of this database, Dr. PIAS has been updated to version 2.0. PPI data have been increased considerably, from 71,500 to 83,324 entries. As the new positive instances in our method, ...

Quantitatively detecting correlations of multiple protein-protein interactions (PPIs) in vivo is a big challenge. Here we introduce a novel method, termed Protein-interactome Footprinting (PiF), to simultaneously measure multiple PPIs in one cell. The principle of PiF is that each target physical PPI in the interactome is simultaneously transcoded into a specific DNA sequence based on dimerizat...

Several large-scale efforts have systematically catalogued protein-protein interactions (PPIs) of a cell in a single environment. However, little is known about how the protein interactome changes across environmental perturbations. Current technologies, which assay one PPI at a time, are too low throughput to make it practical to study protein interactome dynamics. Here, we develop a highly pa...

Protein-protein interactions (PPIs) play important roles in various aspects of the structural and functional organization of cells; thus, detecting PPIs is one of the most important issues in current molecular biology. Although much effort has been devoted to using high-throughput techniques to identify protein-protein interactions, the experimental methods are both time-consuming and costly. I...