conclusions: using n-butanolic c. coum extract in combination with ciprofloxacin offers a novel strategy to control biofilm-based infections caused by p. aeruginosa. results: the ability of biofilm formation by p. aeruginosa strains was quantitatively confirmed. the pcr method confirmed the existence of cup a gene (172 bp) in all studied strains. saponin content of the n-butanolic c. coum extra...

the bacterium pseudomonas aeruginosa is a common cause of infections, and infection with antibiotic-resistant strains can be dangerous. the aim of the present study is to investigate the presence of the exos and exou genes and the formation of biofilms in clinically isolated p.aeruginosa strains. a total of 126 samples were isolated, and p.aeruginosa was identified using biochemical tests. bact...

background: alginate is a linear polysaccharide consisting of guluronate (polyg) and mannuronate (polym) subunits. methods: in the initial screening of alginate-degrading bacteria from soil, 10 isolates were able to grow on minimal medium containing alginate. the optimization of cell growth and alginate lyase (algl) production was carried out by the addition of 0.8% alginate and 0.2-0.3 m nacl ...

Antibiotic resistance has become the main deadly factor in infections, as bacteria can protect themselves by hiding in a self-constructed biofilm. Consequently, more attention is being paid to the search for "non-antibiotic drugs" to solve this problem. Phytoanticipins, the natural antibiotics from plants, could be a suitable alternative, but few works on this aspect have been reported. In this...

results results showed desirable antimicrobial effects of the silver nanoparticles and plant extract on the formation of the pseudomonas aeruginosa biofilm and the highest effect was observed in silver nanoparticles and the lowest inhibitory effects were observed in bucks beard and prangos ferulaceae. conclusions medicinal plants used in the study decreased the rate of biofilm formation. since ...

Background: Some microorganisms can adhere to food handling surfaces forming biofilms that pose a safety challenge. This study was done to evaluate bacterial biofilm formation in milking equipments in Lilongwe, Malawi. Methods: Pooled milk (n=54) and water (n=60) samples were collected from households and milk bulking groups in Lilongwe, Malawi. Swabbing (n=46) and rinsing (n=16) were done on ...

In the present review, we describe and compare the molecular mechanisms that are involved in the regulation of biofilm formation by Pseudomonas putida, Pseudomonas fluorescens, Pseudomonas aeruginosa and Burkholderia cenocepacia. Our current knowledge suggests that biofilm formation is regulated by cyclic diguanosine-5'-monophosphate (c-di-GMP), small RNAs (sRNA) and quorum sensing (QS) in all ...

Background and Aims: Pseudomonas aeruginosa is an opportunistic pathogen. The appearance of the Metallo-β-Lactamases genes and their spread among bacterial pathogens is a matter of concern with regard to the future of antimicrobial therapy.Antimicrobial resistance is an innate feature of bacterial biofilms. Biofilm formation is higher in MDR strains. The present study was undertaken with the ai...

background and objectives: adhesion and colonization are prerequisites for the establishment of bacterial pathogenesis. the biofilm development of pseudomonas aeruginosa was assessed on adhesive surfaces like dialysis membrane, stainless steel, glass and polystyrene. materials and methods: microtiter plate biofilm assay was performed to assess the effect of nutrient medium and growth parameters...