Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real-time investigations dynamic processes vitro and vivo. Here, we propose a field-independent para-hydrogen-based pulsed method to produce rapidly hyperpolarized 13C-labeled substrates. We demonstrate polarizing carboxylic carbon pyruvate moiety purposely designed precur...

Abstract INTRODUCTION Hyperpolarized carbon-13 (HP-13C) MR enables rapid dynamic imaging of metabolic pathways in the human brain using non-toxic, non-radioactive metabolites as tracers. This study presents our unique experience on benefit [1-13C]pyruvate and [2-13C]pyruvate for evaluating patients with glioma. METHODS 132 scans (71 an integrated 13C /1H coil) including steady-state 1H-MRSI (~1...

Purpose To compare carbon-13 (13C) MRSI of hyperpolarized [1-13C]pyruvate metabolism in a murine tumor model with mass spectrometric (MS) imaging the corresponding sections order to cross validate these metabolic techniques and investigate effects pyruvate delivery lactate concentration on labeling. Methods [1-13C]lactate images were obtained from tumor-bearing mice, following injection [1-13C]...

Molecular oxygen is the final electron acceptor in cellular metabolism but cancer cells often become adaptive to hypoxia, which promotes resistance to chemotherapy and radiation. The reduction of endogenous glycolytic pyruvate to lactate is known as an adaptive strategy for hypoxic cells. Whether exogenous pyruvate is required for hypoxic cell proliferation by either serving as an electron acce...

Acetate overflow refers to the metabolism by which a large part of carbon incorporated as glucose into Escherichia coli cells is catabolized and excreted acetate medium. We previously found that mutants for pathway enzymes phosphoacetyltransferase (Pta) kinase (AckA) showed significant diauxic growth after depletion in E. coli. Here, we analyzed underlying mechanism pta mutant. Proteomic other ...

The monocarboxylate pyruvate is an important metabolite and can serve as sole carbon source for Escherichia coli. Although specific pyruvate transporters have been identified in two bacterial species, pyruvate transport is not well understood in E. coli. In the present study, pyruvate transport was investigated under different growth conditions. The transport of pyruvate shows specific activiti...

Protoplasts of the yeast Saccharomyces cerevisiae oxidized externally added pyruvate by pyruvate oxidase system but were not able to decarboxylate it anaerobically by pyruvate decarboxylase at pH 6.4 in isotonic solutions. The decarboxylation set in hypotonic solutions in which the integrity of the plasma membrane was being impaired. Yeast cells incubated with [1-14C]pyruvate accumulated radioa...

The source of hyperpolarized (HP) [(13)C]bicarbonate in the liver during metabolism of HP [1-(13)C]pyruvate is uncertain and likely changes with physiology. Multiple processes including decarboxylation through pyruvate dehydrogenase or pyruvate carboxylase followed by subsequent decarboxylation via phosphoenolpyruvate carboxykinase (gluconeogenesis) could play a role. Here we tested which metab...

2 Introduction 2 Pyruvate formation and metabolisman overview 3 Pyruvate: intracellular antioxidant 5 Oxidative decarboxylation of pyruvate 7 Pyruvate as therapeutic agent 8 Pyruvate detection 9 Summary 9 References 10