1. A theory is developed to account for the kinetics of coupled-enzyme reactions without assuming that the second reaction follows first-order kinetics. 2. A simple procedure is described for applying the theory to the practical design ofenzyme assays. 3. The validity of the theory is confirmed for the assay of glucokinase with glucose 6-phosphate dehydrogenase as coupling enzyme. 4. The possib...

In this theoretical research, the mechanism of the C2H + C2H2 reaction is studied by high-level quantum-chemical methods and kinetics of the reaction is investigated by statistical rate theories. High-level electronic structure calculation methods including M06-2X, CCSD(T), CBS-Q and G4 methods are employed to explore the doublet potential energy surface of the reaction and compute the molecula...

In two-color fluorescence correlation spectroscopy (TCFCS), the fluorescence intensities of two fluorescently-labeled species are cross-correlated over time and can be used to identify static and dynamic interactions. Generally, fluorophore labels are chosen that do not undergo Förster resonance energy transfer (FRET). Here, a general TCFCS theory is presented that accounts for the possibility ...

A reaction kinetic model of the bipolar membrane interface in the bipolar membrane fuel cell (BPMFC) was proposed based on the p-n junction theory and chemical reaction kinetics. It verified the self-humidification feasibility of the BPMFC successfully.

Thomas C. T. Michaelsa, Lucie X. Liua, Samo Curkc, Peter G. Bolhuisd, Anđela Šarićc and Tuomas P. J. Knowlesa,e aDepartment of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK; bPaulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; cDepartment of Physics and Astronomy, Institute for the Physics of Living Systems, University C...

Determine storage capacities, kinetics, and reversibility • for reactions predicted to have high capacity and suitable thermodynamics for H2 storage applications, i.e., 2LiBH4 + 5Mg(BH4)2 and B20H16. Use combined theory and experiment to characterize • reaction products from 2LiBH4 + 5Mg(BH4)2. Synthesize B • 20H16 and determine hydrogen desorption properties and reaction products. Develop comp...

The theory of reaction-diffusion waves begins in the 1930s with the works in population dynamics, combustion theory and chemical kinetics. At the present time, it is a well developed area of research which includes qualitative properties of travelling waves for the scalar reaction-diffusion equation and for system of equations, complex nonlinear dynamics, numerous applications in physics, chemi...

polybutadiene nanoparticles were synthesized via batch emulsion polymerization of butadiene in the presence of potassium persulfate, disproportionate rosinate potassium cation and t-dodecyl mercaptane as initiator, emulsifier and chain transfer agent, respectively. polymerization reaction was performed at different temperatures (60, 70 and 80 °c). conversion was measured at the various time int...