نتایج جستجو برای: recombinant bacmid

تعداد نتایج: 111029  

2013
Asghar Abdoli Hoorieh Soleimanjahi Fatemeh Fotouhi Ali Teimoori Shahram Pour Beiranvand Zahra Kianmehr

OBJECTIVE(S) Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing ant...

Journal: :vaccine research 0
mr shafaati department of cellular & molecular biology iau, hamadan branch, hamadan, iran. e akhavan department of microbiology iau, damaghan branch, damaghan, semnan, iran. sh yazdani department of virology, school of public health, tehran university of medical sciences, tehran, iran. m shafaati department of microbiology iau, jahrom branch, jahrom, shiraz, iran.

introduction: in recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. in this regard, producing a universal vaccine based on virus-like-particles (vlp) could be an ideal approach.  methods: in this study, the full-length orf of influenza hemagglutinin (ha...

Journal: :modares journal of medical sciences: pathobiology 2012
samaneh hossainzadeh fatemeh fotouhi behrokh farahmand maryam saleh atena yousefi

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Journal: :Polish journal of microbiology 2008
Wu-Jie Su Yan Wu Hui-Ling Wu Shan-Ying Zhu Wen-Bing Wang

Open reading frame 76 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm76, is a gene whose function is completely unknown. With EGFP fused to the 3' terminal of Bm76 as the reporter gene and BmNPV bacmid as the expression vector, a recombinant bacmid was successfully constructed expressing Bm76-EGFP fusion protein under the control of polyhedrin promoter in Bombyx mori cells (Bm cel...

2011
S Hossain Zadeh F Fotouhi MT Kheiri MR Razavi B Heydarchi B Farahmand S Najafi

Influenza virus A (H1N1) is an important subtype of influenza virus that makes numerous consequences throughout the world. In the first step of viral attachment, main antigenic site of the HA1 domain from the globular head of hemagglutinin (HA) binds to human cell receptors, starting the disease process. In order to produce recombinant subunit protein vaccines, we focus on the nucleotide sequen...

Background: The importance of viral protein-2 (VP2) of canine parvovirus (CPV) in binding to human cancer cells, production of veterinary vaccines and diagnostic kits has motivated several researches on producing this protein.Objectives: Our purpose was to construct recombinant bacmid shuttle vectors expressing VP2 of CPV using Bac-to-Bac baculoviral expr...

2016
Manoochehr Makvandi Ali Teimoori Niloofar Neisi Alireza Samarbafzadeh

BACKGROUND The hepatitis E virus (HEV) accounts for hepatitis E infection with relatively high mortality rate in pregnant women that can lead to fulminant hepatitis. The baculovirus expression system (BES) has the capability to produce high-level recombinant proteins and could be useful for vaccine designing. OBJECTIVES The aim of this study was designing a recombinant hepatitis E virus ORF2 ...

Journal: :jundishapur journal of microbiology 0
farida behzadian malek ashtar university, research center for sciences and biotechnology, tehran, ir iran zahra goodarzi applied virology research center, baqiyatallah university of medical sciences, tehran, ir iran fatemeh fotouhi influenza research labs, pasteur institute of iran, tehran, ir iran esmaeil saberfar applied virology research center, baqiyatallah university of medical sciences, tehran, ir iran; department of applied virology, baqiyatallah university of medical sciences, tehran, ir iran. tel: +98-2188211523, fax: +98-2188211523

conclusions: the recombinant baculovirus constructed in this work has proper characteristics to produce h5n1 influenza virus-like particles in sf9 cells. results: restriction map of pfastbacihnm1 plasmid confirmed the fidelity of the clone. the pcr carried out on the recombinant bacmids as template indicated that a proper homologous recombination has occurred between pfastbacihnm1 donor plasmid...

Journal: :jundishapur journal of microbiology 0
manoochehr makvandi infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran ali teimoori infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; research center for infectious diseases of digestive system; imam khomeini hospital, ahvaz jundishapur university of medical sciences, ahvaz, ir iran niloofar neisi infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-9166136984, fax: +98-6133332036; +98-6133362411 alireza samarbafzadeh infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

conclusions the results of the present study showed that the baculovirus expression system (sf9 cells) was able to express truncated orf2 and truncated orf2-nsp4 proteins as a potential candidate vaccine. background the hepatitis e virus (hev) accounts for hepatitis e infection with relatively high mortality rate in pregnant women that can lead to fulminant hepatitis. the baculovirus expression...

2015
Xiao-lin Zhang An-min Jiang Zhong-you Ma You-yi Xiong Jin-feng Dou Mei-song Qin Jian-fei Wang

This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori. The signal peptide bombyxin from B. mori was used to promote secretory expression to improve expression levels and was designed and integrated into the UreB gene to generate the Bacmid/BmNPV/(signal peptide)-UreB baculovirus expression system. To determine whethe...

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