نتایج جستجو برای: recombinant coat protein

تعداد نتایج: 1310399  

Journal: :iranian journal of allergy, asthma and immunology 0
naeimeh shibaei department of agricultural biotechnology, national institute of genetic engineering and biotechnology (nigeb), tehran, iran jafar majidi department of immunology, faculty of medicine, tabriz university of medical sciences, tabriz, iran and immunology research center (irc), tabriz university of medical sciences, tabriz, iran khadijeh razavi department of agricultural biotechnology, national institute of genetic engineering and biotechnology (nigeb), tehran, iran ali asghar karkhane department of industrial and environmental biotechnology, national institute of genetic engineering and biotechnology (nigeb), tehran, iran nemat sokhandan-bashir department of plant protection, university of tabriz, tabriz, iran leili aghebati-maleki department of immunology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

Journal: :archives of clinical infectious diseases 0
seyedeh mahya marashiyan department of basic sciences, sari agricultural sciences and natural resources university, sari, iran fatemeh moradian department of basic sciences, sari agricultural sciences and natural resources university, sari, iran geita saadatnia department of biotechnology, iranian research organization for science and technology (irost), tehran, iran; department of biotechnology, iranian research organization for science and technology (irost), tehran, iran. tel/fax: +98-2156276636 majid golkar molecular parasitology laboratory, department of parasitology, pasteur institute of iran, tehran, iran

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

Journal: :Acta biochimica Polonica 2005
Elzbieta Sułuja Ludmiła Strokowskaja Włodzimierz Zagórski-Ostoja Andrzej Pałucha

Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat pr...

Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...

Journal: :Journal of virological methods 2009
Anju Gulati-Sakhuja John L Sears Alberto Nuñez Hsing-Yeh Liu

Pelargonium zonate spot virus (PZSV) is identified recently in tomato plants in the United States. To develop serological diagnostic tools for the detection of this virus, the production of good quality antibodies is a necessity. The coat protein (CP) gene of a California isolate of PZSV was cloned into a bacterial expression vector (pTriEX-4 Ek/LIC). The plasmid pTriEX-4-PZSV-CP was transforme...

Journal: :The Journal of general virology 2002
John V Krondiris Diamantis C Sideris

Here we report on the conformational changes that are responsible for the appearance of the Dicentrarchus labrax encephalitis virus (DlEV) coat protein as a doublet in SDS-PAGE. Wild-type and mutated forms of the coat protein cDNA were expressed in E. coli. The study of the resulting recombinant molecules excluded the possibility of the involvement of a precursor autocatalysis mechanism or a ri...

Journal: :journal of sciences, islamic republic of iran 2011
f. mansouri

a sweet water-soluble protein that reminds stable over wide ranges of temperature and ph, brazzein has various applications. its tastes like cane sugar but have no calories. however, the extraction of brazzein from its natural source is expensive and not applicable. in this study we used recombinant dna technology to provide an alternative option for cheaper mass production of brazzein. a brazz...

Journal: :iranian biomedical journal 0
shreekant m. sapatnekar rajesh k roy rajesh k roy shreekant m. sapatnekar ranjana ranjana a deshmukh

recombinant human interferon alpha 2a (rhifn α -2a) production and cell growth were monitored in a set of genetically modified e. coli strains (msd1519, msd1520, msd 1521, msd 1522, msd 1523) producing rhifn α -2a. the growth was followed at od 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (pyms) of cell biomass and recombinant protein production was determined...

Journal: :jundishapur journal of microbiology 0
mahtab bonyadi department of microbiology, arak university of medical sciences, arak, iran ehsanollah ghaznavi-rad associate professor, department of microbiology, school of medicine, arak university of medical sciences, arak, iran ghasem mosayebi professor entire, department of microbiology and immunology, school of medicine, arak university of medical sciences, arak, iran mohammad rafiei associate professor, department of biostatistics and epidemiology, school of medicine, arak university of medical sciences, arak, iran neda molaee department of microbiology and biotechnology, school of medicine, arak university of medical sciences, arak, iran hamid abtahi associate professor, molecular and medicine research center, arak university of medical sciences, arak, iran; associate professor, molecular and medicine research center, arak university of medical sciences, arak, iran. tel: +98-8634173502, fax: +98-8634173526

conclusions based on the findings, application of recombinant arcaga antigen for the detection of h. pylori infection is a simple, rapid, and brief non-invasive method. therefore, it can be suggested as an appropriate antigen for h. pylori detection kits. results we investigated caga protein, using the immunoblotting method in stool samples, which were already identified as positive by the appl...

Journal: :The Journal of biological chemistry 2004
Ken Sato Akihiko Nakano

Secretory proteins are transported from the endoplasmic reticulum (ER) in vesicles coated with coat protein complex II (COPII). To investigate the molecular mechanism of protein sorting into COPII vesicles, we have developed an in vitro budding reaction comprising purified coat proteins and cargo reconstituted proteolipsomes. Emp47p, a type-I membrane protein, is specifically required for the t...

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