during 2007 – 2008 different fruit gardens in shiraz (fars province, iran) were surveyed for samples being collected from infected apple, pear and quince trees. symptoms of the infected trees included water soaking of infected tissue, wilt and tissue necrosis, scorched and blackend of blossom bracts or young shoots. a total of 47 strains were isolated from infected trees using na medium. on the...

In this study, the suitability of two repetitive-element-based PCR (rep-PCR) assays, enterobacterial repetitive intergenic consensus (ERIC)-PCR and BOX-PCR, to rapidly characterize Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis (CF) was examined. ERIC-PCR utilizes paired sequence-specific primers and BOX-PCR a single primer that target highly conserved repetitive ele...

Different species of Streptomyces are common in most types of soil. Some certain species are plant pathogenic especially on potato. This study was conducted to evaluate genetic diversity among some local Streptomyces spp. strains isolated from soil and infected tubers in potato farms in northwest of Iran. Repetitive DNA elements (BOX, ERIC and REP) as genetic markers were used for diversity stu...

different species of streptomyces are common in most types of soil. some certain species are plant pathogenic especially on potato. this study was conducted to evaluate genetic diversity among some local streptomyces spp. strains isolated from soil and infected tubers in potato farms in northwest of iran. repetitive dna elements (box, eric and rep) as genetic markers were used for diversity stu...

Each year, large volumes of ornamental and food plant propagative stock are imported into the North America; occasionally, Ralstonia solanacearum is found systemically infecting this plant material. In this study, 107 new R. solanacearum strains were collected over a 10-year period from imported propagative stock and compared with 32 previously characterized R. solanacearum strains using repeti...

Repetitive-element PCR (rep-PCR) with primers based on repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) repeated DNA sequences was used for genomic finger-printing of Bartonella species. This technique was applied by using either extracted genomic DNA or preparations of whole bacterial cells directly. PCR fingerprints with either the REP-based p...