in order to investigate the prevalence of infection with bovine viral diarrhoea virus(bvdv) of cattle in ahvaz,blood samples were collected from jugular vein of 572 cattle(521 female, 51 male) from industrial and nonindustrial farms. sera were stored at -20?c while waiting for analysis. the sera were tested by elisa, using a commercial elisa kit for detection of specific antibody to bovine vira...

Several studies suggest that synergisms between Mycoplasma bovis and other microorganisms might exacerbate disease outcome of bovine mycoplasmosis. Screening several bovine cell types to assess their potential use as in vitro infection models for M. bovis, it was observed that a widely used cell line of bovine macrophages (Bomac cells) is in fact persistently infected with bovine viral diarrhea...

Viral infections are ubiquitous in humans, animals, and plants. Real-time methods to identify viral infections are limited and do not exist for use in harsh or resource-constrained environments. Previous research identified that tissues produce unique volatile organic compounds (VOC) and demonstrated that VOC concentrations change during pathologic states, including infection, neoplasia, or met...

mycoplasma and ureaplasma species are the most serious contaminants of cell cultures and this remains one of the major problem encountered in biological research using cell cultures. the aim of this study was to investigate the rate of mycoplasma and a total of 100 different cell culture specimens including, r228, hela, vero, mdck, l20, rd and hep2 were collected. then the specimens were invest...

Recently, a new approach of upstream detection of BVD virus in raw, unprocessed FBS has been described. This is a sandwich enzyme-linked immunosorbent assay (S-ELISA) designed to detect presence of BVD virus in the serum, using a commercially available diagnostic kit (Syracuse Bioanalytical, Syracuse, NY). In this study, the S-ELISA kit that uses raw (unprocessed) FBS as the testing sample was ...

Bovine viral diarrhea virus (BVDV) is one of the most important viral pathogens of cattle worldwide. The aim of present study was to determine the molecular characterization and phylogenetic analysis of BVDV infection in dairy herds of Fars province, Iran. For initial screening, a total of 400 blood samples were collected from 12 industrial dairy herds with previous history of diarrhea, abortio...

Bovine viral diarrhea virus (BVDV) is an RNA belonging to Pestivirus in the family Flaviviridae. BVDV has economic significance for livestock industry because of its association with acute disease, fetal loss, and birth persistently infected (PI) animals. This study aimed investigate infection rates Korean native cattle farms Jeju further planning a control program Province. antibodies antigens...