RNA interference (RNAi) is a biological mechanism that involves the inhibition of gene expression by introduction double-stranded (dsRNA) molecules are complementary to specific target genes. This technique has emerged as promising strategy for management insect pests in crops. The application RNAi pest use dsRNA essential genes species. When these ingested pests, they trigger silencing inhibit...

Background: Introduction of the RNA interference (RNAi) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. In the filamentous fungus Aspergillus nidulans, the gene sidB plays an essential role in septation, conidiation and vegetative hyphal growth. In the present study, we benefited from the RNAi strate...

background: introduction of the rna interference (rnai) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. in the filamentous fungus aspergillus nidulans, the gene sidb plays an essential role in septation, conidiation and vegetative hyphal growth. in the present study, we benefited from the rnai strate...

background: rna interference-based gene silencing has recently been applied as an efficient tool for functional gene analysis. rorc2 is the key transcription factor orchestrating th17 cells differentiation, the cells that are known as the pathogenic elements in various autoimmune diseases. the aim of this study was to design efficient sirnas specific for rorc2 and to evaluate different criteria...

RNA interference (RNAi) is a simple and rapid method for silencing genes in various organisms, it widely used gene function genetics research. RNAi has been developed as novel pest management strategy. Double-stranded RNAs (dsRNAs) delivered via microinjection, ingestion, or soaking are effective insect pests, whereas oral topical delivery methods feasible field applications. Here, we summarize...

conclusions the proliferation and invasion of human bladder cancer cells can be inhibited by rnai-targeting skp2. as a result, skp2 may be a potential target for gene therapy in cases of human bladder cancer. materials and methods the expression of the skp2 gene was knocked down by rna interference (rnai) in t24 cells. the transcription level of skp2 was detected by reverse transcription-quanti...