background: engineering new bone tissue with cells and a synthetic extracellular matrix represents a new approach for the regeneration of mineralized tissues compared with the transplantation of bone (autografts or allografts). methods: in this study, to mimic the mineral and organic component of natural bone, hydroxapatite (ha) and gelatin (gel) composite scaffolds were prepared. the raw mater...

tissue engineering is based on three main factors including scaffolds, cells and growth factors. natural scaffolds derived from decellularized tissues and organs have been successfully used in tissue engineering. decellularization studies have shown that natural scaffolds which maintaine their main structure and properties could be a suitable tool for studying cellular behaviors and preparation...

stem cell research has obtained much prominence in recent years for its therapeutic potential in dealing with serious diseases, many of which are essentially incurable by routine therapies. mesenchymal stem cells with pluripotency and immunomodulatory properties are suitable candidates for tissue engineering and regenerative medicine. today, nanofibrous scaffolds are widely used in tissue engin...

Objective(s): The present study aimed to prepare polycaprolactone (PCL) scaffolds with high porosity and pore interconnectivity, in order to copy the microstructure of natural bones using the thermally induced phase separation (TIPS) technique. Materials and Methods: The scaffolds were coated with chitosan (CH), bioactive glass (BG), and gelatin nanoparticles (GEL NPs) and assessed using ...

Abstract This work introduces the novel gelatin/chitosan blend scaffolds containing different amounts of functionalized multi-walled carbon nanotubes (f-MWCNTs) up to 0.1wt%, which were prepared by freeze drying (freezing and lyophilization). The composite scaffolds were characterized by Fourier transformed infrared spectroscopy (FTIR) to distinguish the functional groups and different bonds in...

objective:more similarity to in vivo medium may help to increase the proliferation and differentiation of cells at in vitro condition. the present study has investigated the effect of a dynamic mediumand nano hydroxyapatite (nha) presence on proliferation and differentiation of mesenchymal stem cells (mscs) to bone cells using electrospun polycaprolactone (pcl) scaffolds. methods: we prepared p...

Background: This study addressed the development of biodegradable and biocompatible scaffolds with enhanced biomechanical characteristics. The biocompatibility and the cationic nature of chitosan (CTS) make it more effective as a bone grafting material. Methods: The hydroxyapatite nanoparticles (nHA) were synthesized by hydrothermal method, and bioglass (nBG) (50% SiO2-45% CaO-5% P2O5) was synt...

currently autografts are gold standards for craniomaxillofacial bone regeneration. but autografts have some limitations. bone tissue engineering has been introduced to overcome these limitations. it consist three components: stem cells, growth factor, and scaffold. scaffold provides an environment for bone growth and simplifies cell adhesion, differentiation and proliferation. in this review, a...